天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (增刊2): 36-43.

• 研究论文 • 上一篇    下一篇

白花三叶草中酚类物质及其抗氧化活性的研究

刘萍,许慧敏,廖鲜艳,王亚茜,黄俊逸*   

  1. 上海大学生命科学学院食品营养与慢性病干预研究室,上海 200444
  • 出版日期:2019-01-18 发布日期:2019-01-18

Studies on Phenolic Compounds and Their Antioxidant Activities in Trifolium Repens

LIU Ping,XU Hui-min,LIAO Xian-yan,WANG Ya-xi,HUANG Jun-yi*   

  1. Laboratory of Food Nutrition and Function,School of Life Sciences,Shanghai University,Shanghai 200444,China
  • Online:2019-01-18 Published:2019-01-18

摘要: 本文测定了白花三叶草不同季节、不同部位的总多酚和总黄酮含量,并比较了它们的抗氧化能力。总多酚和总黄酮含量在不同季节均呈现为叶>叶柄>茎的趋势;而不同部位总黄酮的含量随季节变化不大。DPPH·清除能力和FRAP还原力在不同季节也呈现为叶>叶柄>茎;Rancimat结果显示,各提取物对油脂均具有一定的保护作用。皮尔逊相关系数分析表明总多酚和总黄酮含量与抗氧化活性之间的相关性,肉桂酸、没食子酸和咖啡酸与金雀异黄酮之间的相关性均较好。该研究为开发白花三叶草作为潜在的膳食补充剂和天然抗氧化剂在食品工业中的广泛应用提供了理论基础。

关键词: 白花三叶草, 抗氧化活性, 总多酚, 总黄酮

Abstract: Phenolic compounds content and its antioxidant activities of Trifolium repens in different parts and seasons were performed.Total phenolic content (TPC) and total flavonoid content (TFC) in different parts change in the following order:leaves>stems>shoots in four seasons.However,TFC in different parts change little with the seasons.Notably,the decreased trend of DPPH and FRAP was also in the same order of leaves>stems>shoots in four seasons.The results of Rancimat showed that the extract has a certain protective effect for the oxidantion of oil and fat.Moreover,a strong correlation between TPC,TFC and antioxidant activities indicateed that phenolic compounds as the main components were responsible for the antioxidant behavior of Trifolium repens,and there was a good correlation between cinnamic acid,gallic acid,caffeic acid and genistein.This study implies that Trifolium repens may be a potential dietary supplement and a valuable natural source of antioxidants used in food industries.

Key words: Trifolium repens, antioxidant activity, total phenolic, total flavonoid

中图分类号:  S541.2