广元杜仲与市售不同商品等级杜仲提高免疫和保肝作用的比较研究

doi:10.16333/j.1001-6880.2019.S.002

天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊2): 9-16.doi: 10.16333/j.1001-6880.2019.S.002

广元杜仲与市售不同商品等级杜仲提高免疫和保肝作用的比较研究

熊秋韵，彭成*，高继海，谢晓芳*

成都中医药大学药学院西南特色中药资源国家重点实验室，成都 611137

出版日期:2019-12-28 发布日期:2019-12-30
基金资助:
四川省中央引导地方科技发展专项（2017JZYD0001）；四川省科技厅项目（2017TD0001）；四川省科技厅科技支撑计划项目（2014FZ0121）

Comparative study among commercial Eucommia ulmoides Oliv. of different grades and Eucommia ulmoides Oliv. from Guangyuan on effects of immunity enhancement and liver protection

XIONG Qiu-yun,PENG Cheng*,GAO Ji-hai,XIE Xiao-fang*

Pharmacy College of Chengdu University of Traditional Chinese Medicine,State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China,Chengdu 611137,China

Online:2019-12-28 Published:2019-12-30

摘要/Abstract

摘要： 制备广元精制杜仲、市售杜仲的水提液，以比较其提高免疫、保肝作用的强弱。通过建立氢化可的松、环磷酰胺致免疫低下小鼠模型，观察不同杜仲水提物对碳粒廓清指数K、吞噬指数α、外周血细胞数和免疫器官脏器系数的影响；采用四氯化碳致急性肝损伤小鼠模型，观察不同杜仲水提物对肝脏系数、血清ALT、AST活力和肝组织SOD活力、MDA含量的影响。广元杜仲水提物能明显提升免疫低下小鼠的吞噬指数α（P<0.01）；杜仲水提物均使外周血中PLT含量趋近于正常值，其中二等杜仲对RBC的升高作用明显（P<0.05）；均可降低急性肝损伤模型小鼠血清ALT、AST活力，广元杜仲对ALT的下调作用最佳（P<0.01），而二等杜仲降低AST的作用最显著（P<0.01），均能明显升高肝组织内的SOD活力（P<0.01），二等、三等杜仲可显著减少MDA含量（P<0.01）。综上所述，广元杜仲精制饮片和两种商品等级杜仲均有提高免疫功能和保肝的作用，其中广元杜仲提高免疫功能效果最好，二等杜仲的保肝效果最佳。

关键词: 杜仲, 商品等级, 提高免疫功能, 保肝

Abstract: To prepare water extract of Eucommia ulmoides Oliv. from Guangyuan and commercial Eucommia ulmoides Oliv.(EUO) of different grades,in order to compare the effects on enhancing immune function and protecting liver function among them.Through the immunosuppressive mice models which were made by injecting hydrocortisone subcutaneously,the immunity-enhancement capability of three kinds of EUO was tested by detecting the carbon clearance index K,the phagocytic index α,and the index of immune organs.Through the immunosuppressive mice models which were made by injecting cyclophosphamide subcutaneously,the immunity-enhancement capability of three kinds of EUO was tested by detecting the number of peripheral blood cells and the index of immune organs.The acute liver injury model was induced by injecting CCl₄into mice abdominal cavity,then judge the effect of three kinds of EUO on liver protection by detecting the liver index,the levels of ALT and AST in serum,the SOD and MDA levels in liver tissues.As the result,the water extract of Guangyuan EUO could increase the phagocytic index α of immunosuppressive mice greatly (P<0.01),all water extract of EUO could make PLT counts of immunosuppressive mice tend to be normal,while the second-class EUO obviously increased the RBC counts (P<0.05).Comparing with acute liver injury mice,all drug-treated group had a significant effect on decreasing the levels of ALT and AST in serum,and Guangyuan EUO reduced the ALT level evidently (P<0.01),while the AST level was distinctly lowered by second-class EUO (P<0.01),third-class EUO and Guangyuan EUO (P<0.05).All drug treated groups obviously improved the level of SOD in liver tissues (P<0.01),and decreased MDA level in liver tissues evidently.Second-class and third-class EUO can lower the level of MDA in liver tissues distinctly (P<0.01).In conclusion,EUO from Guangyuan and commercial EUO of different grades all have effects on immunity-enhancement and liver protection.EUO from Guangyuan has the most obvious effect on immunity enhancement,while the second-class EUO’s effect on liver protection was the best.

Key words: Eucommia ulmoides Oliv., commercial grade, immunity enhancement, liver protection

中图分类号:

R285.5

熊秋韵，彭成*，高继海，谢晓芳*. 广元杜仲与市售不同商品等级杜仲提高免疫和保肝作用的比较研究[J]. 天然产物研究与开发, 2019, 31(增刊2): 9-16.

XIONG Qiu-yun,PENG Cheng*,GAO Ji-hai,XIE Xiao-fang*. Comparative study among commercial Eucommia ulmoides Oliv. of different grades and Eucommia ulmoides Oliv. from Guangyuan on effects of immunity enhancement and liver protection[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2019, 31(增刊2): 9-16.

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广元杜仲与市售不同商品等级杜仲提高免疫和保肝作用的比较研究

Comparative study among commercial Eucommia ulmoides Oliv. of different grades and Eucommia ulmoides Oliv. from Guangyuan on effects of immunity enhancement and liver protection

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