天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (1): 30-37.doi: 10.16333/j.1001-6880.2019.1.005

• 研究论文 • 上一篇    下一篇

刺五加内生真菌分离鉴定及抗菌活性研究

刘悦1，张爽1，李佳宾1，沈宏伟1，崔红花2，王振月1*   

  1. 1黑龙江中医药大学，哈尔滨 150040；2广东药科大学中药学院，广州 510006
  • 出版日期:2019-01-28 发布日期:2019-01-28
  • 基金资助:

    “十三﹒五”国家重点研发计划（2016YFC0500303）东北森林区道地林药资源生态开发利用技术研发与示范，黑龙江省国家科技重大专项和重点研发项目（GX17C 006）;黑龙江省博士后资助项目(LBH-Z17208)

Isolation and identification of endophytic fungi from Acanthopanax senticosus (Rupr.Maxim.) harms and their antibacterial activity

LIU Yue1,ZHANG Shuang1,LI Jia-bin1,SHEN Hong-wei1,CUI Hong-hua2,WANG Zhen-yue1*   

  1. 1Heilongjiang University of Chinese Medicine,Harbin 150040,China；2School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006, China
  • Online:2019-01-28 Published:2019-01-28

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摘要： 为开发利用刺五加内生真菌资源，寻找新型抗菌物质，从刺五加根茎叶中分离纯化得到内生真菌71株，经菌落形态和显微形态去重后进行ITS序列分子鉴定为18个种，来自于4目、8科、11属，其中链格孢属与镰孢霉属为优势属。根、茎、叶中内生真菌多样性指数分别为1.112 31、1.523 658、0.264 1。18种刺五加内生真菌中,16种内生真菌至少对一种指示菌有抗菌活性。CWJ-5（镰孢霉属Fusarium oxysporum）对金黄色葡萄球菌抑菌效果显著，抑菌直径27.17±0.07 mm；CWJ-6（链格孢属Alternaria alternata）对大肠埃希菌抑菌效果显著，抑菌直径18.07±0.01 mm。结果表明刺五加内生真菌资源丰富，有很大的开发利用空间，部分菌种抑菌活性显著，值得进一步研究。

关键词: 刺五加, 内生真菌, 鉴定, 多样性指数, 抗菌性

Abstract: In order to develop and use the endophytic fungi resources of Acanthopanax senticosus (Rupr.Maxim.) Harms and to find novel antibacterial substances,71 strains of endophytic fungi were isolated and purified from the roots,stems and leaves of Acanthopanax senticosus (Rupr.Maxim.) Harms.Molecular identification of ITS sequences after decontamination by colony morphology and microscopic morphology,we got 18 species from 4 orders,8 families and 11 genuses,of which Alternaria and Fusarium are dominant genuses.The endophytic fungi diversity index in roots,stems and leaves were 1.123 31,1.523 658,0.264 1,respectively.Among the 18 species of endophytic fungi of Acanthopanax senticosus (Rupr.Maxim.) Harms,16 endophytic fungi have antibacterial activity against at least one indicator bacteria.CWJ-5 (Fusarium oxysporum) has asignificant antibacterial effect against Staphylococcus aureus,with a bacteriostasis diameter of 27.17±0.07 mm;CWJ-6 (Alternaria alternata) has significant antibacterial effect against Escherichia coli.,and its antibacterial diameter can reach 18.07±0.01 mm.The results showed that the endophytic fungi of Acanthopanax senticosus (Rupr.Maxim.) Harms was rich in resources,and there was a great deal of space for development and utilization.Some of the strains had significant antibacterial activity and deserved further study.

Key words: Acanthopanax senticosus (Rupr.Maxim.), endophytic fungi, identification, diversity index, antibacterial

中图分类号: 

R284 R285.5

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