关键词: 钩藤叶, 抗氧化, 绿原酸, 表儿茶素, 芦丁, 金丝桃苷, 喜果苷, 高效液相色谱法

Abstract: The determination method of five active components in the leaves of Uncaria rhynchophylla(Miq.) Miq.ex Havil was established to make up for the deficiency of the quality control method of U.rhynchophylla leaves.In this paper,the antioxidant activity sites of U.rhynchophyllaleaves were traced,and the antioxidant activity of U. rhynchophylla leaves was mainly related to flavonoids and polyphenols.HPLC-PDA method was used to determine the content of five active ingredients such as chlorogenic acid,epicatechin,rutin,hyperoside and vincoside lactam in U. rhynchophylla leaves by gradient elution mode with acetonitrile-0.1% phosphoric acid.The five components were separated well in the chromatographic conditions.The RSDs of the precision,stability and repeatability test were all <3.0%,and the average recoveries were between 96.73%-103.50%.The cluster analysis of 12 batches of samples at different regions showed that the content of the five components was related to geographical distribution of the regions.The established HPLC method is simple in operation,good in accuracy and high in precision,and provides a basis for comprehensive development and utilization of U. rhynchophylla leaves.