关键词: 裸花紫珠, 烧烫伤, 活性部位, 作用机制

Abstract: To screen the active fractions of Callicarpa nudiflora in the treatment of burns and preliminarily study its action mechanism,so as to provide a basis for future research.The scald model of rats was made by 90 ℃ hot water.With the size of the wound,the characteristics of the wound,such as color,degree of hardness and softness,etc.as the indexes,the active parts of Callicarpa nudiflora treatment of burns were screened by macroscopic and microscopic examination.The mechanism of action was preliminarily studied by ELISA and Western blot.After entering the stage of tissue hyperplasia and remodeling,the wound healing of petroleum ether group was the most remarkable than that of other groups;At the final stage of tissue hyperplasia,the epidermis and dermis of petroleum ether group recovered the best and the scar was the smallest.the VEGF concentration in the petroleum ether group was up-regulated significantly in the early stage;In the petroleum ether group,the concentration of VEGF increased significantly in the early stage,and the expression level of TGF-beta 1 was the lowest on the 21st day.The active parts in the treatment of burns were preliminarily speculated to be in the petroleum ether layer.The mechanism of its effect on burns might be that the early stage of the treatment of burns and scalds by Callicarpa nudiflora up-regulated the expression of VEGF and down-regulated the expression of TGF-beta 1 at the later stage.This study provides proofs for further research and application of Callicarpa nudiflora in the treatment of burns.

Key words: Callicarpa nudiflora Hook.et Arn, burns, active parts, functional mechanism