天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (6): 932-939.doi: 10.16333/j.1001-6880.2019.6.002

• 研究论文 • 上一篇    下一篇

枸杞色素及多糖的不同提取次序对其得率及抗氧化活性的影响

李文君1，王成章1*，叶建中1，季金金2   

  1. 1中国林业科学研究院林产化学工业研究所；2南京林业大学化工学院， 南京 210042
  • 出版日期:2019-06-26 发布日期:2019-06-26
  • 基金资助:

    江苏省生物质能源与材料重点实验室项目（JSBEM-S-201810）；林业引智项目（KJZXYZ2018001）

Effect of different extraction order on the yield of Lycium Barbarum pigment and polysaccharides and their antioxidant activities

LI Wen-jun1,WANG Cheng-zhang1*,YE Jian-zhong1,JI Jin-jin2   

  1. 1Institute of Forest Chemical Industry,China Academy of Forestry Sciences; 2Food institute of Nanjing Forestry University,Nanjing 210042,China
  • Online:2019-06-26 Published:2019-06-26

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摘要： 为探究先后提取枸杞多糖及枸杞色素时对各自得率的影响。本研究通过分别考察这两种成分在提取过程中的提取溶剂、提取温度、料液比、提取次数及提取时间等因素对枸杞色素和枸杞多糖的得率影响，确定枸杞色素和枸杞多糖在提取次序不同时，两者的最佳提取工艺以及对DPPH·和·OH自由基清除率。结果表明，首先提取枸杞多糖后，枸杞色素的最佳提取工艺为采用正己烷，80 ℃时，料液比1∶10，提取2次，每次1 h，枸杞色素得率为2.48%，此时枸杞多糖得率为7.45%；而首先提取枸杞色素后，采用了超声辅助提取的方式提取枸杞多糖，发现超声效率为25%，料液比1∶10，提取20 min，枸杞多糖得率为5.23%，此时枸杞色素得率为3.93%。因此，首先提取枸杞多糖，使其平均得率为7.45%，而后提取枸杞色素，其平均得率为2.48%；总体上，枸杞色素1和枸杞多糖1对DPPH·自由基清除率都较高，枸杞多糖1对·OH自由基清除率较高，其抗氧化活性都接近Vc。

关键词: 枸杞多糖, 枸杞色素, 提取次序, 超声辅助, 抗氧化活性

Abstract: In order to explore the influence of different extraction order of  Lycium barbarum polysaccharides and  Lycium barbarum  pigment on their yield,this paper will research respectively the effects of solvent,temperature,ratio of material to liquid,times of extraction and extraction time on the yield of Lycium barbarum polysaccharides and  Lycium barbarum pigment,and the optimum extraction conditions of different order and their free radical scavenging rate of DPPH·and ·OH were determined.The results showed that the best extraction technology of  Lycium barbarum pigment was n-hexane as the solvent,80 ℃,the ratio of material to liquid was 1∶10,extraction time was 1 h,and the yield of pigment was 2.48% and the yield of polysaccharides was 7.45% when extracted polysaccharides as first;the optimum extraction technology of  Lycium barbarum polysaccharides was ultrasonic efficiency as 25%,the ratio of material to liquid was 1∶10,and extracted for 20 min with ultrasonic assisted extraction,and the yield of polysaccharides was 5.23% and the yield of polysaccharides was 3.93% when extracted pigment as first.Consequently,the average yield of  Lycium barbarum polysaccharide was 7.45%,and pigment was 2.48% when firstly extracted polysaccharide.In general,Lycium barbarum pigment 1 and  Lycium barbarum polysaccharide 1 have higher free radical scavenging rate of DPPH·and  Lycium barbarum polysaccharide 1 has higher free radical scavenging rate of ·OH ,and their antioxidant activities were close to Vc.

Key words: Lycium barbarum polysaccharides, Lycium barbarum pigment, extraction order, ultrasound assisted, antioxidant activity

中图分类号: 

R284.2 R917

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