天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊1): 40-46.doi: 10.16333/j.1001-6880.2019.S.008

• 研究论文 • 上一篇    下一篇

5种秦艽组植物近红外判别模型的建立

李佩佩1, 2,李朵1, 2,栾真杰1, 2,孟晓萍1,周玉碧1,孙菁1*   

  1. 1青海省青藏高原特色生物资源研究重点实验室 中国科学院西北高原生物研究所,西宁 810008;2中国科学院大学,北京 100049
  • 出版日期:2019-08-06 发布日期:2019-08-06
  • 基金资助:

    青海省重点实验室发展建设专项(2017-ZJ-Y10);国家自然科学基金青年基金(81403051);青海省自然科学基金面上项目(2019-ZJ-904);青藏高原特色生物资源工程技术研究中心能力建设项目(2017-GX-G03)

Establishment of near-infrared discriminant model of five Cruciata Gaudin

LI Pei-pei1,2, LI Duo1,2, LUAN Zhen-jie1,2, MENG Xiao-ping1, ZHOU Yu-bi1, SUN Jing1*   

  1. 1Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resource, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; 2University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2019-08-06 Published:2019-08-06

摘要: 本文以5种秦艽植物为研究对象,建立正品秦艽与非正品秦艽(模型1)及不同秦艽物种(模型2)的近红外判别模型,并与聚类分析比较。以模型1为代表进行建模条件优化,经优化,利用一阶导数谱图经discriminant analysis建模,两个模型预测率与识别率均达到100%,可对正品秦艽与非正品秦艽及样品种类进行准确判别。利用5种植物的吸光度进行聚类分析,结果,聚类分析能准确判别正品秦艽与非正品秦艽,但无法对秦艽种类判别,表明近红外判别模型比聚类分析具有更广泛的实用性及准确度,可为秦艽药材临床用药的安全性和有效性提供快速鉴别的科学依据。

关键词: 麻花艽, 达乌里秦艽, 秦艽, 粗茎秦艽, 黄管秦艽, 近红外判别模型

Abstract: Setting the 5 Gentiana species-G. straminea, G. dahurica, G. macrophylla, G. crassicaulis, G. officinalis-as the research objects. Near-infrared combined with chemometrics were used to build two discriminant models. Model 1 was to discriminant authentic Radix Gentiana Macrophyllae from falsify;model 2 was to distinguish 5 different species. And the cluster analysis was also proceeded to make a comparison. Model 1 was taken to optimize modeling approaches and spectral processing methods. Finally, two models were established by discriminant analysis, with no-smoothing first-derivative spectra. And the recognition rates and prediction rates of two models were all 100%. The NIR absorbance was analyzed by cluster analysis. The result showed that cluster analysis could discriminant authentic gentiana from adulterants exactly, but could not identify 5 different species. The analysis indicated that discriminant models have more practicability and accuracy. They can provide a rapid and scientific method on clinical medication safety and effectiveness of Radix Gentiana Macrophyllae.

Key words: G. straminea, G. dahurica, G. macrophylla, G. crassicaulis, G. officinalis, near-infrared discriminate model

中图分类号: 

O657.33