天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊2): 67-72.

• 研究简报 • 上一篇    下一篇

黑玫瑰水果玉米花青素积累特性、稳定性及生物活性研究

宋肖敏婷1,2,陈雪3,王洁1,2,陈媛媛1,杨林1,刘倩倩1,2,陈文俊3*,陈江1,2*


  

  1. 1成都中医药大学药学院;2中药资源系统研究与开发利用国家重点实验室培育基地,成都 611137;3成都农业科技职业学院特种玉米研究所,成都611130

  • 出版日期:2020-12-09 发布日期:2020-12-09
  • 基金资助:
    四川省科技厅项目(18YYJC0083)

Study on accumulation characteristics,stability and biological activity of anthocyanin in Black Rose Fruit Maize

SONG Xiao-min-ting1,2,CHEN Xue3,WANG Jie1,2,CHEN Yuan-yuan1,YANG Lin1,LIU Qian-qian1,2,CHEN Jun-wen3*,CHEN Jiang1,2*   

  1. 1Pharmacy College,Chengdu Univeity of Traditional Chinese Medicine;2State Key Laboratory Breeding Base of Research and Development and Utilization of Traditional Chinese Medicine Resources System,Chengdu 611137,China;3Chengdu Institute of Agricultural Science and Technology,Special Corn Research Institute,Chengdu 611130,China

  • Online:2020-12-09 Published:2020-12-09

摘要:

以自选黑玫瑰水果玉米品种(黑甜玉8号)为材料,采用分光光度计法和四甲基偶氮唑盐比色法,分析花青素积累特性、稳定性及生物活性。结果表明:黑玫瑰水果玉米在授粉后27、33天花青素含量高于授粉后21天,且玉米籽粒中花青素含量高于其他组织部位;其籽粒经冻干处理提取的花青素含量较高,整体稳定性高于烘干和煮熟处理;温度、pH和氧化还原剂对花青素稳定性都有一定影响;其叶片、茎秆、穗轴、苞叶部位花青素提取物能抑制人B淋巴瘤细胞OCI-LY10和人急性淋巴髓单核细胞白血病MV4-11的增殖,半数抑制浓度(50% inhibitory concentration,IC50)分别为144.2、82.8、123.8、95.6 μg/mL和58.8、12.6、43.0、49.4 μg/mL。

关键词: 黑玫瑰水果玉米, 花青素, 积累特征, 稳定性, 生物活性

Abstract:

The Black Rose Fruit Maize variety (Black and sweet maize No.8) is used as the material.The anthocyanin accumulation characteristics,stability and biological activity were analyzed by spectrophotometry and tetrazolium salt colorimetry assay.The results showed that the content of anthocyanin at 27 th and 33th day after pollination was higher than that at day 21,and the content of anthocyanin in maize kernel was much higher than other tissues;the content of anthocyanin extracted by lyophilization treatment of Black Rose Fruit Maize kernels was higher than baking and boiling.Temperature,pH and redox agent have an influence on the stability of anthocyanins.The anthocyanin extract from the leaf,stem,core and shell can inhibit the proliferation of human B lymphoma cell line OCI-LY10 and human acute lymphoblastic monocytic leukemia MV4-11,and the IC50 was 144.2,82.8,123.8,95.6 μg/mL and 58.8,12.6,43.0,49.4 μg/mL.

Key words: Black Rose Fruit Maize, anthocyanin, accumulation feature, stability, biological activity

中图分类号:  R931.6