关键词: 蒲公英, &alpha, -葡萄糖苷酶, 气相色谱-质谱联用, 分子对接, 网络药理学

Abstract: In this study,a screening model of α-glucosidase inhibitor in vitro was utilized to screen the activity of the extract of Taraxacum mongolicum Hand.-Mazz from Guizhou Province.The results showed that the petroleum ether extracts of the extract of T. mongolicum from Guizhou Province significantly inhibited the activity of α-glucosidase (IC₅₀=0.9 μg/mL).And its activity was stronger than that of the positive drug acarbose (IC₅₀=174.3 μg/mL).Thirty-seven compounds were isolated and identified from petroleum ether extracts of T. mongolicum from Guizhou by GC-MS.Five small molecule compounds with strong drug-forming ability were obtained after virtual screening.Two small molecular compounds were identified as have have good affinity with human alpha-glucosidase after molecular docking with human α-glucosidase .Based on network pharmacology,the "hypoglycemic component-target" map of dandelion was constructed to explain the hypoglycemic mechanism of dandelion.

Key words: Taraxacum mongolicum Hand.-Mazz, α-glucosaccharase;gas chromatography-mass spectrometry;molecular docking;network