基于铁离子作用结合高效液相色谱在线筛选金花茶花黄酮中抗氧化物质和体内抗氧化活性研究

doi:10.16333/j.1001-6880.2020.5.001

天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (5): 719-726.doi: 10.16333/j.1001-6880.2020.5.001

• 研究论文 • 下一篇

基于铁离子作用结合高效液相色谱在线筛选金花茶花黄酮中抗氧化物质和体内抗氧化活性研究

张海龙1,2，虞青婷1，吴清孝1，秦小明1*

1广东海洋大学食品科学与技术学院广东省水产加工与安全重点实验室广东省亚热带果蔬加工现代农业科技创新中心，湛江 524088；2怀化学院生物与食品工程学院，怀化 418008

出版日期:2020-05-28 发布日期:2020-06-16
基金资助:
亚热带果蔬加工与利用技术研究（2013050214）

On-line screening antioxidant components based on iron binding-HPLC and antioxidant activity in vivo of Camellia nitidissima Chi flower flavonoids

ZHANG Hai-long1,2,YU Qing-ting1,WU Qing-xiao1,QIN Xiao-ming1*

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1College of Food Science and Technology,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, and Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing,Guangdong Ocean University,Zhanjiang 524088,China;2College of Biological and Food Engineering,Huaihua University,Huaihua 418008,China

Online:2020-05-28 Published:2020-06-16

摘要/Abstract

摘要： 为了研究金花茶花黄酮的抗氧化效果，首先采用基于铁离子作用结合高效液相色谱的方法在线筛选金花茶花黄酮的抗氧化物质，并用清除DPPH和ABTS自由基方法验证潜在组分的抗氧化效果，其次用时间飞行质谱表征抗氧化物质，最后结合体内抗氧化的方法评估金花茶茶花黄酮抗氧化性活性。通过铁离子作用结合高效液相色谱法快速筛选出9种抗氧化物质，分别为表儿茶素或儿茶素、原花青素二聚体、原花青素三聚体、原花青素四聚体、芹菜素-6-C-葡萄糖-8-C-木糖苷、芦丁、异槲皮素或异槲皮素同分异构体。体内抗氧化研究表明金花茶花黄酮能显著提升大鼠体内过氧化氢酶（60.47±2.8 U/mg）、谷胱甘肽过氧化物酶（649.1±51.5 U/mg）和总超氧化物歧化酶（69.91±4.56 U/mg）含量，降低脂质过氧化物丙二醛（1.83±0.16 nmol/mg）含量，改善大鼠体内氧化应激效应。

关键词: 金花茶花黄酮, 铁离子作用结合高效液相色谱, 在线筛选, 抗氧化

Abstract:

Antioxidant components in Camellia nitidissima Chi flower flavonoids (Cnfe) were studied with an on-line screening method based on iron binding-HPLC,and off-line antioxidant methods (DPPH and ABTS).The compounds with potential antioxidant effects in Cnfe was identified using a Time of Flight Mass Spectrometry (TOF/MS) method.In addition,the antioxidant activity in vivo of Cnfe was also investigated.Nine compounds in the Cnfe were found to possess the potential antioxidant activity.They were identified as epicatechin,procyanidin dimer,procyanidin C1,procyanidin tetramer,6-C-pentosyl-8-C-hexosyl apigenin,rutin and quercetin-glucoside.The results of in vivo antioxidant activity showed that Cnfe significantly increased the content of catalase (60.47±2.80 U/mg),glutathione peroxidase (649.10±51.50 U/mg) and total superoxide dismutase (69.91±4.56 U/mg),and reduced the content of malondialdehyde (1.83±0.16 nmol/mg).Thus Cnfe improved the oxidative stress in rats.

Key words: Camellia nitidissima Chi flowers flavonoids, iron binding-HPLC, on-line screening method, antioxidant

中图分类号: TS201

张海龙, 虞青婷, 吴清孝, 秦小明. 基于铁离子作用结合高效液相色谱在线筛选金花茶花黄酮中抗氧化物质和体内抗氧化活性研究[J]. 天然产物研究与开发, 2020, 32(5): 719-726.

ZHANG Hai-long, YU Qing-ting, WU Qing-xiao, QIN Xiao-ming. On-line screening antioxidant components based on iron binding-HPLC and antioxidant activity in vivo of Camellia nitidissima Chi flower flavonoids[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2020, 32(5): 719-726.

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基于铁离子作用结合高效液相色谱在线筛选金花茶花黄酮中抗氧化物质和体内抗氧化活性研究

On-line screening antioxidant components based on iron binding-HPLC and antioxidant activity in vivo of Camellia nitidissima Chi flower flavonoids

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