天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (5): 778-782.doi: 10.16333/j.1001-6880.2020.5.008

• 研究简报 • 上一篇    下一篇

阔鳞鳞毛蕨黄酮类化学成分的分离与鉴定

王林1，李焕勇1，梁妮2，陈能花3*   

  1. 1暨南大学分析测试中心，广州 510632；2珠海市斗门区新盈中等职业学校，珠海 519100；3暨南大学中药及天然药物研究所，广州 510632

  • 出版日期:2020-05-28 发布日期:2020-06-16
  • 基金资助:
    国家自然科学基金（81973190）

Isolation and identification of the flavonoids constituents from Dryopteris championii

WANG Lin1,LI Huan-yong1,LIANG Ni2,CHEN Neng-hua3*   

  1. 1Analytical and Testing Center,Jinan University,Guangzhou 510632,China;2Zhu Hai Xinying Secondary Vocational School,Zhuhai 519100,China;3Institute of Traditional Chinese Medicine & Natural Products,Jinan University,Guangzhou 510632,China

  • Online:2020-05-28 Published:2020-06-16

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摘要： 为研究阔鳞鳞毛蕨Dryopteris championii的化学成分，本研究利用硅胶、ODS、Sephadex LH-20柱层析及制备型高效液相等色谱分离技术对阔鳞鳞毛蕨地上部分的95%乙醇提取物进行分离纯化，从乙酸乙酯萃取部位分离得到13个黄酮类化合物，并通过现代光谱和文献对比的方法鉴定其结构分别为表儿茶素（1）、木犀草素（2）、柚皮素（3）、圣草酚（4）、槲皮素（5）、芹菜素（6）、山奈酚（7）、黄芪苷（8）、广寄生苷（9）、槲皮素-3-O-β-D葡萄糖苷（10）、牡荆素（11）、芹菜素-7-O-β-D-葡萄糖苷（12）、槲皮素-3-O-6-反式-对香豆酰基-β-D-葡萄糖-(1→2)-α-L-鼠李糖苷（13），包括1个黄烷醇、2个二氢黄酮、4个黄酮、6个黄酮醇。化合物1～13均为首次从该种植物中分离得到，其中6、8、9、11和13为首次从该属植物分离得到。


关键词: 阔鳞鳞毛蕨, 化学成分, 黄酮, 木犀草素, 柚皮素 

Abstract:

In order to study the chemical constituents from Dryopteris championii,the isolation and purification of the 95% EtOH extract of the aboveground part of D. championii were carried out with various chromatographic separation techniques of silica gel,ODS,Sephadex LH-20,as well as the preparative high performance liquid chromatography.It led to the isolation of thirteen flavonoids compounds of which structures were determined by spectral analysis and comparison with literature data.They were identified as (-)-epicatedchin (1),luteolin (2),naringenin (3),eriodictyol (4),quercetin (5),apigenin (6),kaempferol (7),astragalin (8),avicularin (9),quercetin-3-O-β-D-glucopyranoside (10),vitexin (11),apigenin-7-O-β-D-glucopyranoside (12),and quercetin-3-O-6-E-p-coumaroyl-β-D-glucopyranosyl-(1→2)-α-L-rhamnopyranoside (13),including one flavanol,two flavanones,four flavones,and six flavonols. Among them,all of the compounds were isolated from this plant for the first time,and of which compounds 6,8,9,11 and 13 were obtained from the genus of Dryopteris for the first time.

Key words: Dryopteris championii, chemical constituents, flavonoids, luteolin, naringenin

中图分类号:  R284.2

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