天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (6): 989-994.doi: 10.16333/j.1001-6880.2020.6.011

• 研究简报 • 上一篇    下一篇

黔产徐长卿根茎化学成分及抗烟草花叶病毒活性

唐攀1，陈洁1，晏英1,2，胡嘉琪2，徐冉1,2，张吉泉1,2，汤磊1,2*   

  1. 1贵州医科大学药学院，贵阳 550025；2贵州医科大学 贵州省化学合成药物研发利用工程技术研究中心，贵阳 550004 

  • 出版日期:2020-06-28 发布日期:2020-07-06
  • 基金资助:
    国家自然科学基金（31760089）；贵州省自然科学基金（黔科合基础［2017］1141）；贵阳市科技局计划（筑科合同［2017］30-28）

Chemical consitituents and anti-TMV activity of the roots and stems of Cynanchum paniculaun in Guizhou Province

TANG Pan1,CHEN Jie1,YAN Ying1,2,HU Jia-qi2,XU Ran1,2,ZHANG Ji-quan1,2,TANG Lei1,2*   

  1. 1College of Pharmacy,Guizhou Medical University,Guiyang 550025,China; 2Engineering Technology Research Center for Chemical Drug R&D,Guizhou Medical University,Guiyang 550004,China

  • Online:2020-06-28 Published:2020-07-06

PDF (PC)

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摘要：

通过应用多种色谱方法从徐长卿根茎的乙醇提取物中分离得到13个单体化合物，运用现代波谱技术鉴定了结构，分别为glaucogenin A（1）、glaucogenin A 3-O-β-D-cymaropyranoside（2）、N-(N-benzoyl-S-phenylalaninyl)-S-phenylalaninol acetate（3）、N-(N-benzoyl-S-phenylalaninyl)-S-phenylalaninol benzoate（4）、androst-4,6-dien-3,17-dione（5）、20-hydroxy-4,6-diene-gestrol-3-one（6）、5,7-dihydroxy-6-methoxyflavone（7）、5,7-dihydroxy-8-methoxyflavone（8）、5-(hydroxymethyl)-1-isopentyl-1H-pyrrole-2-carbaldehyde（9）、mono-terpenes（10）、4-hydroxy-3,5-dimethoxybenzoic acid（11）、pavonisol（12）、4-hydroxy-3,5-dimethoxy-benzoic acid（13）。其中化合物2~5和7~10系首次从该植物中分离得到。离体叶圆片法和钝化活性测试表明，化合物1~4和9均有抗TMV活性，保护活性测试表明，化合物4具有抗TMV活性，且首次发现化合物3、4、9具有抗TMV活性。

关键词: 徐长卿, 化学成分, 分离纯化, 抗TMV活性

Abstract:

Thirteen compounds were obtained from the ethanol extract of the roots and stems of Cynanchum paniculatum by applying various chromatographic techniques.Their structures were elucidated and identified as glaucogenin A (1),glaucogenin A 3-O-β-D-cymaropyranoside (2),N-(N-benzoyl-S-phenylalaninyl)-S-phenylalaninol acetate (3),N-(N-benzoyl-S-phenylalaninyl)-S-phenylalaninol benzoate (4),androst-4,6-dien-3,17-dione (5),20-hydroxy-4,6-diene-gestrol-3-one (6),5,7-dihydroxy-6-methoxyflavone (7),5,7-dihydroxy-8-methoxyflavone (8),5-(hydroxymethyl)-1-isopentyl-1H-pyrrole-2-carbaldehyde (9),mono-terpenes (10),4-hydroxy-3,5-dimethoxybenzoic acid (11),pavonisol (12),4-hydroxy-3,5-dimethoxy-benzoic acid (13),respectively,based on the spectral data.Among them,compounds 2-5 and 7-10 were obtained from the fruits of C. paniculatum for the first time.The results showed that compounds 1-4 and 9 have anti-TMV activity,through in vitro disc method and inactivation effect tested.The protection effect test showed that compound 4 has anti-TMV activity.It was found that compound 3,4 and 9 have anti-TMV activity for the first time.

Key words: Cynanchum paniculatum, chemical constituents, separation and purification, anti-TMV activity

中图分类号:  R284.1 R932

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