天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (8): 1379-1388.doi: 10.16333/j.1001-6880.2020.8.014

• 开发研究 • 上一篇    下一篇

紫叶李叶黄酮提取工艺的响应面优化及最佳采收月份的确定

甄可妙1，苏琪1，闫子涵1，国洪宾1，高剑1，李梦冉2，周程艳1*


  

  1. 1河北大学药学院，河北省药物质量分析控制重点实验室，保定 071002；2沧州师范学院生命科学学院，沧州 061000

  • 出版日期:2020-08-28 发布日期:2020-09-02
  • 基金资助:
    河北省科技计划（B2015201016）；保定市科学技术研究与发展指导计划（18ZF121）

Optimization of extraction of flavonoids from Prunus cerasifera leaves using response surface methodology and determination of optimum harvest month

ZHEN Ke-miao1,SU Qi1,YAN Zi-han1,GUO Hong-bin1,GAO Jian1,LI Meng-ran2,ZHOU Cheng-yan1*#br#

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  1. 1College of Pharmaceutical Science,Key Laboratory of Pharmaceutical Quality Control of Hebei Province,Hebei University,Baoding 071002,China;2College of life sciences,Cangzhou Normal University,Cangzhou 061000,China

  • Online:2020-08-28 Published:2020-09-02

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摘要：

使用高效液相色谱仪测定不同条件下超声辅助提取紫叶李叶中矢车菊素-3-O-葡萄糖苷花青素、芦丁、槲皮素、山奈酚4种黄酮类物质的得率，并根据4种物质的活性，建立紫叶李叶中黄酮类物质得率权重计算公式。在单因素试验的基础上，采用Box-Behnken设计-响应面法优化紫叶李叶黄酮类物质提取工艺。应用最佳提取工艺处理不同月份紫叶李叶，并根据4种成分权重计算黄酮类物质得率，确定最佳采收月份。结果表明，紫叶李叶黄酮类物质最佳提取工艺为乙醇体积分数63.93%、液料比25∶1（mL/g）、提取温度44.08 ℃、提取时间41.62 min；最佳采收月份为8月份。此结果重复性好，可信度高，其中矢车菊素-3-O-葡萄糖苷花青素得率随时间变化最明显，且与日照条件显著相关，该试验对后期相关具体成分的提取具有借鉴意义。

关键词: 紫叶李叶, 黄酮类物质, 响应面法, 高效液相色谱, 超声辅助提取

Abstract:

The yield of ultrasonic assisted extraction of cyanidin-3-O-glucoside,rutin,quercetin and kaempferol in the leaves of Prunus cerasifera were determined by high-performance liquid chromatography (HPLC). And according to the activity of the four substances,the weight calculation formula for the yield of flavonoids in the leaves of P. cerasifera was established. On the basis of single-factor test,Box-Behnken response surface methodology was used to optimize extraction process of flavonoids from P. cerasifera leaves. The leaves of P. cerasifera in different months were treated by the best extraction process,and the flavonoids yield was calculated according to the weight of four substances to determine the best picking month. The results showed that the optimum extraction conditions were as follows:ethanol volume fraction was 63.93%,ratio of liquid to raw material was 25∶1 (mL/g),extraction temperature was 44.08 ℃,and extracted for 41.62 min. And the best picking month was August. The results showed good repeatability and high reliability. The yield of cyanidin-3-O-glucoside changed most obviously over time,and was also significantly related to sunshine conditions. All in all,the study in this paper can provide the beneficial reference for the extraction of related components in the future.

Key words: Prunus cerasifera , leaves, flavonoids, response surface methodology, HPLC, ultrasonic assisted extraction

中图分类号:  TQ464

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