天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (8): 1389-1395.doi: 10.16333/j.1001-6880.2020.8.015

• 开发研究 • 上一篇    下一篇

大别山白及多糖酶法辅助提取及活性研究

朱富成，罗书岚，郑宣，王芳，何晓梅，邓辉，韩邦兴*   

  1. 皖西学院 生物与制药工程学院 安徽省中药资源保护与利用工程实验室，六安 237012

  • 出版日期:2020-08-28 发布日期:2020-09-02
  • 基金资助:
    安徽省自然科学基金（2008085QB96）；国家中药材产业技术体系（CARS-21）；安徽省教育厅重点项目（KJ2017 A407）；皖西学院博士后科研项目（WXBS H2019001）

Enzyme-assisted extraction of polysaccharides of Ta-pieh Mountains Bletilla striata and its activities study

ZHU Fu-cheng,LUO Shu-lan,ZHENG Xuan,WANG Fang,HE Xiao-mei,DENG Hui,HAN Bang-xing*#br#

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  1. College of Biology and Pharmaceutical Engineering,Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources,West Anhui University,Luan 237012,China

  • Online:2020-08-28 Published:2020-09-02

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摘要：

白及多糖具有显著的药用价值和良好的护肤作用。本研究首次利用果胶酶酶解的方法，提高白及多糖提取效率。首先，通过响应面优化法，获取最佳酶解条件，在最优条件下大别山白及多糖提取率达64.8%。随后，利用扫描电镜观察和空间构象分析表明酶法提取没有破坏多糖天然结构。体外性质研究表明在一定浓度下白及多糖对羟基自由基、DPPH和超氧阴离子的清除率分别到达87.6%、83.9%和71.5%，显示了较好的抗氧化活性。本研究中的白及多糖提取率为已报道文献的最高量，为白及多糖工业化应用奠定基础，且为其他多糖的高效提取提供参考。

关键词: 大别山白及, 白及多糖, 酶法提取, 响应面法, 抗氧化性

Abstract:

Bletilla striata polysaccharides (BSP) have attracted extensive research interest due to their potential application of medicinal value and skin care. Herein,enzymatic assisted technique was applied to extract BSP from B. striata of Ta-pieh Mountains for the first time. Firstly,response surface methodology was applied to improve extraction efficiency. Under the optimal conditions,the maximum extraction yield was 64.8%. Scanning electron microscope and spatial conformation analysis shown that enzymatic extraction did not destroy the natural structure of polysaccharides. Then,antioxidant activity of extracted BSP was analyzed,and the results clearance rates of hydroxyl radical,superoxide anion and DPPH were 87.6%,71.5% and 83.9%,respectively,at the finite concentration of BSP,which presented a good antioxidant activity. The extraction yield of BSP was highest report to data. The efficient extraction of the BSP in this study lays the foundation for its industrial application.

Key words: Bletilla striata of Ta-pieh Mountains, Bletilla striata , polysaccharides, enzyme-assisted extraction, response surface methodology, antioxidant activity

中图分类号:  R284.2

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