天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 109-115.doi: 10.16333/j.1001-6880.2020.S.018

• 开发研究 • 上一篇    下一篇

石珊瑚层藻多糖的提取及其体外抗氧化活性研究

孙妍1,2,3，蒋芳3，侯域3，俞伟1，潘道东2*，周海滨1*   

  1. 1宁波立华制药有限公司，宁波 315174；2宁波大学浙江省动物蛋白食品精深加工技术重点实验室，宁波 315211；

  • 出版日期:2020-06-28 发布日期:2020-07-20

Study on the extraction of polysaccharide from Lithothamnion superpositum and its antioxidant activity in vitro

SUN Yan1,2,3 JIANG Fang3,HOU Yu3,YU Wei1,PAN Dao-dong2*,ZHOU Hai-bin1*#br#

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  1. 1Ningbo Lihua Pharmaceutical Co.,Ltd.,Ningbo 315174,China;2 Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province,Ningbo University,Ningbo 315211,China;3Zhejiang Pharmaceutical College,Ningbo 315100,China

  • Online:2020-06-28 Published:2020-07-20

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摘要： 为实现进口石珊瑚层藻多糖的高效提取，本文分别采用热水法、超声波辅助法提取粗多糖，并对其方法学进行了系统的评价。同时对所得粗多糖的体外抗氧化活性进行了分析。结果表明，传统热水法的粗多糖得率（9.83%）较超声法（8.47%）高，但其含有较多的蛋白和多酚，致使其多糖含量（64.71%）较超声法（65.55%）低。体外抗氧化实验结果表明，超声提取法所得的粗多糖对DPPH自由基具有较高的清除效率。本研究明确了超声法相较于热水法更适合提取石层珊瑚藻多糖，为石层藻多糖的高效提取及多糖质量评价提供方法学支撑和数据支持。


关键词: 石珊瑚层藻, 多糖, 提取, 抗氧化

Abstract:

In order to achieve the high-efficient extraction of polysaccharide from Lithothamnion superpositum,the hot water method and ultrasonic assisted method were used to extract the polysaccharide,and the methodology was systematically evaluated.Meanwhile the antioxidant activity of the crude polysaccharide in vitro was analyzed.The results showed that the yield of crude polysaccharide (9.83%) of traditional hot water method was higher than that of ultrasonic method (8.47%),but it contained more protein and polyphenol,resulting in the content of polysaccharide (64.71%) lower than that of ultrasonic method (65.55%).The results of antioxidant experiments in vitroshowed that the polysaccharide extracted by ultrasound had a high scavenging efficiency on DPPH free radicals.To sum up,this study confirmed that ultrasonic method is more suitable to extract polysaccharide from coralline than hot water method,which provides methodological support and data support for the efficient extraction and quality evaluation of Polysaccharide from coralline.

Key words: Lithothamnion superpositum, polysaccharide, extraction, antioxidation

中图分类号:  R917

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