天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 77-84.doi: 10.16333/j.1001-6880.2020.S.013

• 研究简报 • 上一篇    下一篇

汉桃叶茎和叶的挥发油化学成分差异GC-MS分析

晏婷1,3,蒯晴叶1,3,黄勇1,蒲健4,郑林1,李勇军2,王爱民2*   

  1. 1贵州医科大学贵州省药物制剂重点实验室 药用植物功效与利用国家重点实验室;2贵州医科大学民族药与中药开发应用教育部工程研究中心;3贵州医科大学药学院;4贵州益佰制药股份有限公司 贵阳 550004

  • 出版日期:2020-06-28 发布日期:2020-07-20
  • 基金资助:
    国家重点研发计划(2018YFC1708100);国家自然科学基金(81960763);贵州省科技计划(黔科合平台人才[2019]5407/5660,[2017]5601);贵阳市科研创新团队项目(筑科合同[2017]30-29号)

GC-MS analysis of chemical constituents of volatile oil from stems and leaves of Schefflera arboricola Hagata

YAN Ting1,3,KUAI Qing-ye1,3,HUANG Yong1,PU Jian4,ZHENG Lin1,LI Yong-jun2,WANG Ai-min2*   

  1. 1Key Laboratory of Pharmaceutical Preparations of Guizhou Province/Guangzhou Medical University State Key Laboratory of Medicinal Plant Efficacy and Utilization;2Engineering Research Center of National Medicine and Traditional Chinese Medicine 
    Development and Application Ministry of Education,Guizhou Medical University;3College of Pharmacy,Guizhou Medical University;4Guizhou Yibai Pharmaceutical Co.,Ltd.,Guiyang 550004,China

  • Online:2020-06-28 Published:2020-07-20

摘要:

通过比较汉桃叶茎和叶部位中挥发油的化学成分,为汉桃叶茎和叶的药效物质基础提供实验依据。采用水蒸气蒸馏法提取汉桃叶茎、叶部位的挥发油,并用GC-MS法对其化学成分进行分析与鉴定,通过峰面积归一化法得出各成分在组分中的相对含量,比较不同部位的化学成分。从汉桃叶茎和叶挥发油中共鉴定出86种挥发油化学成分。茎中挥发油化学成分鉴定出50种(46.01%),其挥发油化学成分相对含量排在前三位的依次为[1aR-(1aα,4aα,7β,7aβ,7bα)]-1氢-1,1,7-三甲基-4-亚甲基-1氢-环丙基 [e] 甘菊环-7-醇(5.62%),石竹烯氧化物(5.45%),胡椒烯(1.98%);叶中挥发油化学成分鉴定出58种(60.74%),其挥发油化学成分相对含量排在前三位的依次为1a,2,3,4,4a,5,6,7b-8氢-1,1,4,-四甲基-[1aR-(1aα,4α,4aβ,7bα)]-1氢-环丙基[e]薁(11.79%);顺-β-金合欢烯(8.80%);[1aR-(1aα,4aα,7β,7aβ,7bα)]-1氢-1,1,7-三甲基-4-亚甲基-1氢-环丙基[e]甘菊环-7-醇(6.77%)。汉桃叶茎和叶的挥发油共有化学成分22种,主要相对含量高的是[1aR-(1aα,4aα,7β,7aβ,7bα)]-1氢-1,1,7-三甲基-4-亚甲基-1氢-环丙基[e]甘菊环-7-醇(5.62%,6.77%)。因此,汉桃叶茎和叶挥发油化学成分具有一定的差异性,在应用时需加以注意,本研究为汉桃叶药材的进一步药用提供参考。

关键词: 汉桃叶, 挥发油, 不同提取部位, GC-MS

Abstract:

By comparing the chemical constituents of volatile oils obtained from the stem and leaf parts of Schefflera arboricolaHagata,it provides an experimental basis for the medicinal substance basis of the stem and leaf of S. arboricola.The steam oil distillation method was used to extract the volatile oil from stems and leaves of S. arboricola and a GC-MS method was used to analyze and identify its chemical composition.The relative content of each component in the constituents was obtained using peak area normalization method,and the chemical components of different parts were compared.A total of 86 volatile oil chemical constituents were identified from the stem and leaf of S. arboricola.In the stems,50 species (46.01%) of volatile oil chemical components were identified,and its relative contents of volatile oil components ranked in the top three were [1aR-(1aα,4aα,7β7aβ,7bα)]-1hydrogen-1,1,7-trimethyl-4-methyl-1H-cyclopropyl[e] chamomile-7-ol(5.62%),caryophyllene oxide (5.45%), and piperene (1.98%).Moreover,58 species (60.74%) of volatile oil constituents were identified in the leaves,and the relative contents of volatile oil constituents ranked in the top three were 1a,2,3,4,4a,5,6,7b-octahydro-1,1,4,7-tetramethyl-[1aR-(1aα,4α,4aβ,7bα)]-1H-cycloprop[e]azulene(11.79%),cis-β-farnesene (8.80%),and [1aR-(1aα,4aα,7β,7aβ,7bα)] -1hydrogen-1,1,7-trimethyl-4-methylene-1H-cyclopropyl[e] chamomile-7-ol (6.77%).There were 22 common constituents of volatile oil from the stem and leaf parts of S. arboricola,and the main high relative content both was [1aR-(1aα,4aα,7β,7aβ,7bα)]-1 hydrogen-1,1,7-trimethyl-4-methylene-1H-cyclopropyl[e]chamomile-7-ol (5.62%,6.77%). Therefore, the volatile oils compositions of leaves and stems of S. arboricola has certain differences,and it should be paid attention in clinical application.This study provides a reference for the further medicinal use of S. arboricola.

Key words: Schefflera arboricola , Hagata, volatile oil, different extraction sites, GC-MS

中图分类号:  R932