天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊2): 105-115.

• 开发研究 • 上一篇    下一篇

不同前处理方法提取墨红花香成分的对比研究

王辉1,喻文娟2,奉树成1*


  

  1. 1上海植物园/上海城市植物资源开发应用工程技术中心,上海 200231;2上海交通大学 分析测试中心,上海 200240

  • 出版日期:2020-12-09 发布日期:2020-12-09
  • 基金资助:
    上海市科学技术委员会项目(18DZ2283500)

Comparative study on extraction of the flower aroma components of ‘Crimson Glory’ by different pretreatment methods

WANG Hui1,YU Wen-juan2,FENG Shu-cheng1*#br#

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  1. 1Shanghai Botanical Garden/Shanghai Engineering Research Center of Sustainable Plant Innovation,Shanghai 200231,China;2Instrumental Analysis Center,Shanghai Jiaotong University,Shanghai 200240,China

  • Online:2020-12-09 Published:2020-12-09

摘要:

墨红是已开发的既可食用又可香料用的芳香月季的典型代表。本研究以墨红为实验材料,采用几种方法提取和分析其鲜花香气成分,以探索适合芳香月季的香气前处理方法。结果显示三种方法共检出138种成分,固相微萃取(SPME)检出68种,占92.58%,成分主要分布色谱峰的前、中段;搅拌子吸附萃取脱附(SBSE-TD)技术检出80种,占92.51%,成分在色谱峰的前中后段都有;吹扫捕集-脱附(P&T-TD)技术检出97种,占79.22%,色谱峰的覆盖范围和SBSE-TD差不多,峰密度更高,无香和杂质成分也比较多。三种方法检出醇类化合物成分相对含量都是最高的,主要香气类型为花香。三种分析方法各具特点,从便捷性,资源筛选和开发的角度来看,SBSE-TD法更具优势。

关键词: 墨红, 香气成分, 提取, 分析, 前处理

Abstract:

‘Crimson Glory’ is a typical representative of the aromatic rose that has been developed for both edible and perfumery.In this study,‘Crimson Glory’ was used as experimental material to extract and analyze the aroma components of fresh flower by several methods in order to explore the aroma pretreatment method suitable for aromatic rose.The results showed that 138 components were total detected by the three methods.Sixty-eight components (92.58%) were detected by solid phase microextraction (SPME),the main components of which were distributed in the front and middle of the chromatographic peak,and the efficiency of detecting the aroma active components was high.eighty species (92.51%) were detected by stir bar sorptive extraction-thermal desorption (SBSE-TD),and the components were found in the front,middle and back of the chromatographic peak,and the efficiency of detecting active aroma components was also high.Ninety-seven components (79.22%) were detected by purge and trap-thermal desorption (P&T-TD).The coverage range of chromatographic peaks was about the same as that of SBSE-TD,the density of chromatographic peaks was higher than that of SBSE-TD,but the efficiency of detecting active aroma components was lower and there were more aroma free and impurity components.From the categories of compounds detected,the three methods are similar,and the relative content of alcohols is the highest,and the main aroma type is flower fragrance.The three analysis methods have their own characteristics,and SBSE-TD method has more advantages from the point of view of convenience,resource screening and development.

Key words: Crimson Glory, aroma components, extraction, analysis, pretreatment

中图分类号:  R284.1