天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (1): 79-88.doi: 10.16333/j.1001-6880.2021.1.011

• 开发研究 • 上一篇    下一篇

青藏高原黑果枸杞花青素对人肝癌细胞增殖和自噬的影响

王梦杰1，李玉林2，刘嘉华1，张龙飞1，阿孝蓉1，周佳俊1，吴华1*，柴沙驼3*   

  1. 1青海大学农牧学院；2中国科学院西北高原生物研究所；3青海大学畜牧兽医科学院，西宁 810000

  • 出版日期:2021-01-28 发布日期:2021-01-28
  • 基金资助:
    青海省科技厅应用基础研究计划项目（2018-ZJ-759）

Effect of anthocyanidin of Lycium ruthenicum Murry from Qinghai Tibet Plateaun on the proliferation and autophagy of HepG2 cells

WANG Meng-jie1,LI Yu-lin2,LIU Jia-hua1,ZHANG Long-fei1,A Xiao-rong1,ZHOU Jia-jun1,WU Hua1*,CHAI Sha-tuo3*   

  1. 1College of Agriculture and Animal Husbandry,Qinghai University;2Institute of Northwest Plateau Biology,Chinese Academy of Sciences;3Qinghai Academy of Science and Veterinary Medicine of Qinghai University,Xining 810000,China

  • Online:2021-01-28 Published:2021-01-28

PDF (PC)

127

摘要：

探究黑果枸杞花青素在体外对人肝癌HepG2细胞增殖和自噬的影响。利用CCK-8法测定细胞活力，EdU和细胞划痕试验检测细胞增殖和迁移效果，RT-PCR和Western blot检测增殖和自噬相关基因的mRNA和蛋白表达。结果显示，黑果枸杞花青素可有效抑制人肝癌HepG2细胞的增殖和迁移；上调增殖因子（LATS1、LATS2和MOB1）和自噬因子（Beclin-1、LC3-Ⅱ和AMPK），并下调增殖因子YAP的mRNA水平；下调自噬因子p-mTOR和细胞周期因子CDK4，并上调自噬因子p-AMPK和LC3-Ⅱ的蛋白表达。由此推测黑果枸杞花青素可在体外抑制人肝癌HepG2细胞增殖和迁移，并促进人肝癌HepG2细胞发生自噬。

关键词: 黑果枸杞花青素, 人肝癌HepG2细胞, 增殖, 自噬

Abstract:

To explore the effect of Lycium ruthenicum Murray anthocyanidin on proliferation and autophagy of HepG2 cells in vitro,the viability of HepG2 cells were detected by CCK-8.Besides,HepG2 cells proliferation and migration were explored by EdU and cell scratch test.Then RT-PCR and Western blot were performed to explore the expression of proliferation-related genes and autophagy-related genes at mRNA and protein level,respectively.We found that L. ruthenicum anthocyanidin could significantly inhibit the proliferation and migration of HepG2 cells.The mRNA expression of proliferation factors (LATS1,LATS2 and MOB1) and autophagy factors (Beclin-1,LC3-Ⅱ and AMPK) induced by  L. ruthenicum anthocyanidin were up-regulated,while the mRNA expression of proliferation factor YAP induced by  L. ruthenicum anthocyanidin were down-regulated.Furthermore, L. ruthenicum anthocyanidin decreased autophagy factor p-mTOR and cyclin factor CDK4 protein expression,while enhanced autophagy factors (p-AMPK and LC3-Ⅱ) protein expression.In conclusion,L.ruthenicum anthocyanidin can inhibit the proliferation and migration,and promote the autophagy of HepG2 cells in vitro.

Key words: Lycium ruthenicum , Murray anthocyanidin, HepG2 cells, proliferation, autophagy

中图分类号:  R932

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：