天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (11): 1894-1900.doi: 10.16333/j.1001-6880.2021.11.012

• 开发研究 • 上一篇    下一篇

霍山石斛菌根真菌多样性及差异性的研究

刘丽1,2,陈绍通3,张腾2,李琳2,刘旭宇2,韩邦兴2,4*   

  1. 1安徽中医药大学药学院,合肥 230031;2皖西学院生物与制药工程学院,六安 237012;3华南农业大学,广州 510000;4安徽省中药资源保护与持续利用工程实验室,六安 237012

  • 出版日期:2021-11-28 发布日期:2021-12-02
  • 基金资助:
    国家“十三五”重点研发计划(2017YFC1700701);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-21);安徽省科技重大专项(202003a07020003);安徽省高校学科(专业)拔尖人才学术项目(gxbjZD 2020083)

Diversity and difference of mycorrhizal fungi in Dendrobium huoshanense

LIU Li1,2,CHEN Shao-tong 3,ZHANG Teng 2,LI Lin2,LIU Xu-yu2,HAN Bang-xing2,4*   

  1. 1College of Pharmacy,Anhui Universty of Chinese Medicine,Hefei 230031,China;2College of Biological and Pharmaceutical Engineering,West Anhui University,Lu’an 237012,China;3South China Agricultural University,Guangzhou 510000,China;4Anhui Engineering Laboratory of Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources,Lu’an 237012,China

  • Online:2021-11-28 Published:2021-12-02

摘要: 运用Illumina MiSeq高通量测序方法对霍山石斛菌根真菌ITS1-2区进行测序,采用Mothur分析三种类型的一年生霍山石斛菌根真菌的菌群多样性、差异性及群落结构组成。结果显示,霍山石斛样品中共得到898 663条ITS rRNA序列,1 928个OTUs;三种类型的一年生霍山石斛(ⅠR1、ⅡR1、ⅢR1)菌根真菌的平均Shannon指数分别为2.42、2.79、2.74;菌根真菌多样性丰富,主要包括子囊菌门、担子菌门、毛霉门三个门,其中5月份霍山石斛中子囊菌门的丰度最大,担子菌门的丰度最小;LEfSe分析发现三种类型的一年生霍山石斛菌根真菌有44个差异丰富的分类学分支(a = 0.01,LDA Score≥2.0)。

关键词: 霍山石斛, 菌根真菌, 高通量测序, 多样性

Abstract:

In order to analyze the diversity and structure of mycorrhizal fungi in three types of Dendrobium huoshanense,the ITS 1-2 region of D. huoshanense mycorrhizal fungi was sequenced by Illumina Miseq high-throughput sequencing method and analyzed by mothur.The results showed that 898 663 ITS rRNAs and 1 928 OTUs were obtained from three types of annual D. huoshanense.The average Shannon index of mycorrhizal fungi of three types (ⅠR1,ⅡR1 and ⅢR1) were 2.42,2.79 and 2.74,respectively.Investigations on D. huoshanense revealed high richness and diversity of mycorrhizal fungi,mainly including Ascomycota,Basidiomycota and Mucoromycota phylum,among which the abundance of Ascomycota was the highest in May,and that of Basidiomycota was the lowest.LEfSe analysis showed that there were 44 different branches (a = 0.01,LDA score ≥ 2.0) among the three types of annual D. huoshanense mycorrhizal fungi.

Key words: Dendrobium huoshanense, mycorrhizal fungi, high-throughput sequencing, diversity

中图分类号:  R932