天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (增刊2): 1-6.

• 研究论文 •    下一篇

油橄榄叶甲醇提取物多酚成分、抗氧化及抑癌活性研究

黄智，罗思源，刘莉，陈涛，丁春邦，冯士令*   

  1. 四川农业大学生命科学学院，雅安 625014
  • 出版日期:2021-12-28 发布日期:2021-11-22
  • 基金资助:
    四川省科技计划（2020YFH0207）

Study on polyphenols,antioxidant and anticancer activities of methanol extract from olive leaves

HUANG Zhi,LUO Si-yuan,LIU Li,CHEN Tao,DING Chun-bang,FENG Shi-ling*   

  1. College of Life Science,Sichuan Agricultural University,Ya'an 625014,China
  • Online:2021-12-28 Published:2021-11-22

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摘要： 以油橄榄叶为材料，利用HPLC测定其甲醇提取物（EOL）中多酚成分，并通过秀丽线虫和Hela细胞探究其抗氧化及抗肿瘤活性。结果表明，EOL中含芦丁、木犀草素-4′-O-葡萄糖苷、芹菜素-7-O-葡萄糖苷、木犀草素和芹菜素5种主要多酚成分。EOL显著提高秀丽线虫抗氧化酶SOD、CAT和GSH-Px的活性，降低MDA水平，使其氧化应激存活率提升了72.52%。此外，EOL通过上调Caspase 9和Caspase 3活性抑制Hela细胞增殖，且肿瘤细胞抑制率呈现浓度和时间依赖型。因此，EOL具有开发为抗氧化和抑癌药物的潜力。

关键词: 油橄榄叶, 多酚, 秀丽线虫, 抗氧化活性, Hela细胞

Abstract:

The methanol extract of olive leaves (EOL) was obtained by ultrasonic-assisted method.The polyphenols of EOL were determined by HPLC,and they antioxidant and anti-tumor activities were explored by Caenorhabditis elegans and Hela cells.The results showed that EOL contains 5 main polyphenol components including rutin,luteolin-4′-O-glucoside,apigenin-7-O-glucoside,luteolin and apigenin.EOL significantly increased the antioxidant enzyme activities of SOD,CAT and GSH-Px,decreased the level of MDA,and increased the survival rate of C. elegans under oxidative stress by 72.52%.In addition,HeLa cells were treated with different EOL concentrations to determine the inhibition rate of HeLa cells and the underlying anti-tumor mechanism of EOL was also explored.The results showed that EOL inhibited the proliferation of Hela cells by up-regulating the activities of Caspase 9 and Caspase 3,and the tumor cells inhibition rate was concentration and time-dependent.Therefore,EOL has the potential to be developed as an antioxidant and anticancer drugs.

Key words: olive leaf, polyphenols, C. elegans, antioxidant activity, Hela cells

中图分类号:  Q949.9

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