天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (10): 1727-1735.doi: 10.16333/j.1001-6880.2022.10.011

• 研究简报 • 上一篇    下一篇

基因工程菌Streptomyces bingchenggensis BCJ60的次级代谢产物研究

李建宋1，李   亚2*，郝之奎1，张明涛1，王继栋3，向文胜4   

  1. 1台州职业技术学院医学与制药工程学院；2浙江海正药业股份有限公司，台州 318000；3湖州师范学院生命科学学院，湖州 313000；4东北农业大学生命科学学院，哈尔滨 150030
  • 出版日期:2022-10-28 发布日期:2022-10-31
  • 基金资助:
    台州市科技计划（21gyb27）；浙江省教育厅一般科研项目（Y202044775）；台州职业技术学院博士基金（2021BS02）

Secondary metabolites from the genetically engineered strain Streptomyces bingchenggensis BCJ60

LI Jian-song1,LI Ya2*,HAO Zhi-kui1,ZHANG Ming-tao1,WANG Ji-dong3,XIANG Wen-sheng4   

  1. 1School of Medicine and Pharmaceutical Engineering,Taizhou vocational and technical college;2Zhejiang Hisun Pharmaceutical Co.,Ltd.,Taizhou 318000,China;3College of Life Science,Huzhou University,Huzhou 313000,China;4School of Life Science,Northeast Agricultural University,Harbin 150030,China
  • Online:2022-10-28 Published:2022-10-31

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摘要：

对基因工程菌Streptomyces bingchenggensis BCJ60的次级代谢产物化学成分及杀虫活性进行研究，采用正相硅胶柱层析、反向硅胶柱层析和Sephadex LH-20分离纯化，结合核磁共振、质谱等波谱学方法鉴定化合物结构，从中分离获得11个化合物，分别为3,4-dihydro-3-hydroxy-5-oxomilbemycin A3（1）、3,4-dihydro-3-hydroxy-5-oxomilbemycin A4（2）、seco-milbemycin C（3）、seco-milbemycin A（4）、米尔贝霉素H（5）、米尔贝霉素β13（6）、米尔贝霉素ST906（7）、米尔贝霉素β3（8）、25-ethylmilbemycin β3（9）、米尔贝霉素β6（10）和米尔贝霉素β7（11）。其中化合物1和2为新化合物，杀虫活性结果显示，化合物1和2对朱砂叶螨（LC50 = 0.121 ± 0.010 mg/L和0.116 ± 0.015 mg/L）和松材线虫（LC50 = 5.217 ± 0.064 mg/L和5.581 ± 0.059 mg/L）具有显著的杀虫活性，与商品化的杀虫剂米尔贝霉素A3/A4相比没有显著性差异。

关键词: 基因工程菌, Streptomyces bingchenggensis BCJ60, 次级代谢产物, 杀虫杀螨活性

Abstract:

To study the chemical constituents from a culture of the genetically engineered strain Streptomyces bingchenggensis BCJ60.All compounds were isolated and purified by various column chromatographies,including silica gel,Rp-18 and Sephadex LH-20,and their structures were elucidated through the interpretation of NMR and MS spectroscopic data.Eleven milbemycin derivatives were isolated from methanol extract of Streptomyces bingchenggensis BCJ60,which were identified as 3,4-dihydro-3-hydroxy-5-oxomilbemycin A3 (1),3,4-dihydro-3-hydroxy-5-oxomilbemycin A4 (2),seco-milbemycin C (3),seco-milbemycin A (4),milbemycin H (5),milbemycin β13 (6),milbemycin ST906 (7),milbemycin β3 (8),25-ethylmilbemycin β3 (9),milbemycin β6 (10) and milbemycin β7 (11).Compounds 1 and 2 were new compounds.Compounds 1 and 2 possessed potent activities against Tetranychus cinnabarinus (LC50 = 0.121 ± 0.010 mg/L and 0.116 ± 0.015 mg/L) and Bursaphelenchus xylophilus (LC50 = 5.217 ± 0.064 mg/L and 5.581 ± 0.059 mg/L),which were comparable to those of commercial pesticide milbemycins A3/A4.

Key words: genetically engineered strain, Streptomyces bingchenggensis BCJ60, secondary metabolites, acaricidal and nematocidal activities

中图分类号:  R284.1

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