天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (6): 1014-1020.doi: 10.16333/j.1001-6880.2022.6.014

• 开发研究 • 上一篇    下一篇

紫花前胡苷通过调控CXCL5-ERK/MEK信号轴发挥抗肝癌作用

王鹏利,王国泰,闫曙光,李京涛,罗   璐,白   垚,周小燕*   

  1. 陕西中医药大学附属医院,咸阳 712000
  • 出版日期:2022-06-28 发布日期:2022-06-30
  • 基金资助:
    咸阳市2019年重点研发计划(2019k02-100)

Nodakenin exerts anti-hepatocellular carcinoma effects by regulating CXCL5-ERK/MEK signal axis

WANG Peng-li,WANG Guo-tai,YAN Shu-guang,LI Jing-tao,LUO Lu,BAI Yao,ZHOU Xiao-yan*   

  1. Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China
  • Online:2022-06-28 Published:2022-06-30

摘要:

本文探讨紫花前胡苷(nodakenin,NK)对肝癌细胞的作用及机制。不同浓度紫花前胡苷处理HepG2和MHCC-97H细胞,CCK-8检测细胞增殖变化,细胞划痕和Trans-wells实验观察细胞迁移和侵袭能力变化。紫花前胡苷能够显著抑制HepG2和MHCC-97H细胞增殖、体外迁移和侵袭;RNA-seq分析紫花前胡苷给药前后各基因表达,qRT-PCR、Western blotting和细胞免疫荧光进行验证分析,结果表明紫花前胡苷给药后趋化因子5(CXCL5)的表达显著降低,同时ERK和MEK磷酸化被抑制。综上所述紫花前胡苷通过下调CXCL5抑制ERK/MEK信号通路从而发挥抗肝癌作用。

关键词: 紫花前胡苷, CXCL5, ERK/MEK, 肝癌

Abstract:

This paper investigates the effect and mechanism of nodakenin (NK) on hepatocellular carcinoma cells.HepG2 and MHCC-97H cells were treated with different concentrations of NK.Cell proliferations were detected by CCK-8,and migration and invasion abilities were observed by cell scratch and Trans-wells experiments.NK significantly inhibited the proliferation,migration and invasion of HepG2 and MHCC-97H cells in vitro.RNA-seq was conducted to analyze the expression of each gene before and after the administration of NK.qRT-PCR,Western blotting and cellular immunofluorescence were used to verify the RNA-seq result.The results showed that the expression of chemokine 5 (CXCL5) was significantly decreased after administration of NK,and the phosphorylation of ERK and MEK was inhibited at the same time.In summary,it can be concluded that NK inhibits ERK/MEK signaling pathway by down-regulating CXCL5,thereby inhibiting hepatocellular carcinoma.

Key words: nodakenin, CXCL5, ERK/MEK, liver cancer

中图分类号:  R932