天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (9): 1539-1547.doi: 10.16333/j.1001-6880.2022.9.011

• 开发研究 • 上一篇    下一篇

箭叶淫羊藿多糖新组分EP80的结构表征及其对酒精代谢相关酶活力的影响研究

张梦园1，杨晓华2,3，段梦月1,4，张华峰1,3*   

  1. 1陕西师范大学食品工程与营养科学学院 中俄食品与健康科学国际联合研究中心 西北濒危药材资源开发国家工程实验室，西安 710119；2西安交通大学医学部，西安 710061；3云南省普洱市院士专家工作站，普洱 665600；4安徽粮食工程职业学院，合肥 230012
  • 出版日期:2022-09-28 发布日期:2022-10-09
  • 基金资助:
    国际科技合作项目（GJ2021-27）；陕西省重点研发计划（2018ZDXM-SF-006，2020ZDLSF05-03，2022ZDLSF05-11）

Structural characterization of novel fraction EP80 of Epimedium sagittatum polysaccharides and its effects on enzymes related to alcohol metabolism

ZHANG Meng-yuan 1,YANG Xiao-hua 2,3,DUAN Meng-yue 1,4,ZHANG Hua-feng 1,3*   

  1. 1National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China,International Joint Research Center of Shaanxi Province for Food and Health Sciences,College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,China;2Health Science Center,Xi'an Jiaotong University,Xi'an 710061,China;3Academician and Expert Workstation in Puer City of Yunnan Province,Puer 665600,China;4Anhui Vocational College of Grain Engineering,Hefei 230012,China
  • Online:2022-09-28 Published:2022-10-09

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摘要：

采用乙醇分级分离技术从箭叶淫羊藿中分离得到了多糖新组分EP80，应用薄层色谱、热重分析等方法分析了EP80的理化性质，应用傅里叶变换红外光谱、圆二色谱、刚果红实验、扫描电子显微镜等技术研究了EP80的结构特征，考察了EP80的体外抗氧化活性及其对酒精代谢相关酶的效应。结果表明，EP80由半乳糖、阿拉伯糖、葡萄糖和木糖组成，其分子量为3.2×104 Da，具有吡喃糖苷骨架，含有三股螺旋结构，在230 nm附近有正Cotton效应，在260 nm附近有负Cotton效应，微观形貌呈不规则片状，有突出的褶皱结构，热失重区间主要集中在240~350 ℃。EP80清除DPPH和ABTS自由基的半有效浓度（EC50）分别为0.029和0.235 mg/mL。EP80对乙醇脱氢酶和乙醛脱氢酶均具有激活作用，对乙醛脱氢酶的EC50明显低于乙醇脱氢酶（P＜0.01）。本研究为淫羊藿多糖新组分开发利用提供了参考依据。

关键词: 箭叶淫羊藿, 多糖组分EP80, 化学结构, 抗氧化, 乙醛脱氢酶, 乙醇脱氢酶

Abstract:

A novel fraction of polysaccharides EP80 was prepared from Epimedium sagittatum by grading-alcohol precipitation.Physicochemical properties of fraction EP80 were analyzed by different techniques such as thin-layer chromatography and thermogravimetric analysis.Structural characteristics of fraction EP80 were studied by various methods such as Fourier transform infrared spectroscopy,circular dichroism,Congo red experiment and scanning electron microscopy.Besides,in vitro antioxidant activity and effects of fraction EP80 on two enzymes related to alcohol metabolism were investigated.The results indicate that fraction EP80 with triple helix structure has a pyranose-ring skeleton.And its molecular weight was 3.2×104 Da,which was composed of galactose,arabinose,glucose and xylose.There are a positive Cotton effect near 230 nm and a negative Cotton effect near 260 nm.The micromorphology of fraction EP80 is irregular flakes with prominent fold structure.Fraction EP80 thermally loses weight at the range of 240-350 ℃.The effective concentrations (EC50) that scavenge DPPH and ABTS radicals by 50% are 0.029 and 0.235 mg/mL,respectively.Fraction EP80 obviously activates alcohol dehydrogenase and acetaldehyde dehydrogenase,and its EC50 on acetaldehyde dehydrogenase is significantly lower than that on alcohol dehydrogenase (P＜0.01).These results are helpful to exploitation and utilization of novel fractions of E. sagittatum polysaccharides.

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中图分类号:  R284

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