天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (9): 1548-1556.doi: 10.16333/j.1001-6880.2022.9.012

• 开发研究 • 上一篇    下一篇

一株罗布泊盐湖链霉菌的鉴定及其代谢产物对酪氨酸酶活性、黑色素生成的抑制效应

王俊1,2,3，付建红1,2,3*，王玉苗1,2,3，阮文伟1,2,3，聂慧林1,2,3，崔凤真1,2,3   

  1. 1新疆特殊环境物种保护与调控生物学实验室；2新疆师范大学干旱区植物逆境生物学实验室；3新疆师范大学生命科学学院，乌鲁木齐830054
  • 出版日期:2022-09-28 发布日期:2022-10-09
  • 基金资助:
    新疆维吾尔自治区自然科学基金（2021D01A122）

Identification of a Streptomyces setonii from Lop Nur and inhibition effects of its metabolites on tyrosinase activity and melanin synthesis

WANG Jun1,2,3,FU Jian-hong1,2,3*,WANG Yu-miao1,2,3,RUAN Wen-wei1,2,3,NIE Hui-lin1,2,3,CUI Feng-zhen1,2,3   

  1. 1Xinjiang Key Laboratory of Special Species Conservation and Regulation Biology;2Key Laboratory of Plant Stress Biology in Arid Land,Xinjiang Normal University;3College of Life Science,Xinjiang Normal University, Urumqi 830054,China
  • Online:2022-09-28 Published:2022-10-09

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摘要：

本研究采用多相分类法对一株分离自新疆罗布泊盐湖链霉菌89-2-2进行鉴定，通过CCK-8法、L-多巴氧化法和NaOH溶解法检测该菌株乙酸乙酯提取物对小鼠黑色素瘤B16细胞增殖、细胞内酪氨酸酶活性及黑色素含量的影响。以抑制酪氨酸酶活性为指标，应用LC-MS代谢组学方法检测链霉菌89-2-2发酵条件优化前后所产生的差异代谢物，分析可能具有抑制活性的代谢产物类型。结果初步鉴定该菌株为西唐氏链霉菌（Streptomyces setonii）89-2-2。S. setonii  89-2-2乙酸乙酯提取物在100~1 000 μg/mL浓度范围内几乎无细胞毒性，但能有效抑制B16细胞内酪氨酸酶活性和黑色素生成，与空白组相比均具有显著性差异（P < 0.05）。代谢组学实验的结果显示S. setonii 89-2-2产生的差异代谢物主要为维生素类化合物、芳香类化合物和羧酸类化合物。本研究为从新疆罗布泊链霉菌中开发酪氨酸酶抑制剂和黑色素合成抑制剂提供重要的科学依据。

关键词: 罗布泊链霉菌, 代谢产物, 酪氨酸酶抑制活性, 小鼠黑色素瘤B16细胞, 黑色素合成抑制活性

Abstract:

In this study,a Streptomyces sp. 89-2-2 isolated from Lop Nur Salt Lake in Xinjiang was identified by multiphase classification.Effects of the strain ethyl acetate extract on mouse melanoma B16 cell proliferation,tyrosinase activity and melanin content were determined by CCK-8 assay,L-Dopa oxidation method and NaOH dissolution method.Taken the inhibitory activity on tyrosinase as evaluation index,we detected the differential metabolites produced by Streptomyces sp. 89-2-2 under the two fermentation conditions of before and after optimization by liquid chromatograph-mass spectrometer.The aim of this study was to analyze the types of metabolites that may have inhibitory activity.The strain was preliminary identified as Streptomyces setonii 89-2-2.The ethyl acetate extract of S. setonii 89-2-2 had little cytotoxic,but could effectively inhibit the tyrosinase activity and melanogenesis in the B16 cells under the concentration of 100-1 000 μg/ml.The above results were significantly different from those of the blank groups (P < 0.05).Metabolomics test results showed that the differential metabolites produced by S. setonii 89-2-2 were mainly vitamins,aromatic compounds and carboxylic acids.This study provides an important scientific basis for the development of tyrosinase inhibitors and melanin synthesis inhibitors from Streptomycesof Lop Nur in Xinjiang.

Key words: Streptomyces setonii from Lop Nur, metabolites, inhibitory activity on tyrosinase, mice melanoma B16 cell, inhibitory activity on melanin synthesis

中图分类号:  Q936

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