天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (增刊2): 57-61.

• 研究简报 • 上一篇    下一篇

滇西北地区车前草抗氧化活性研究

苏林军1,4,杨耀权1,4,杨晓燕1,2,3,4,佘   容1,4*   

  1. 1大理大学 天然抗氧化剂和抗氧化炎症研究院;2中国三江并流区域生物多样性协同创新中心;3大理大学 三江并流区域生物多样性保护与利用云南省创新团队;4大理大学 东喜玛拉雅研究院,大理 671003
  • 出版日期:2022-12-07 发布日期:2022-12-07
  • 基金资助:
    云南省科技厅科技计划(202101BA070001-115);第二次青藏高原综合科学考察研究资助项目(2019QZKK0402)

Study on antioxidant activity of plantain grown in northwestern Yunnan

SU Lin-jun1,4,YANG Yao-quan1,4,YANG Xiao-yan1,2,3,4,SHE Rong1,4*   

  1. 1Institute of Natural Antioxidants and Antioxidant Inflammation,Dali University;2Collaborative Innovation Center for Biodiversity in Three Parallel Rivers of China;3Yunnan Provincial Innovation Team for Biodiversity Conservation and Utilization in Three Parallel Rivers,Dali University;4Institute of Eastern-Himalaya Biodiversity Research,Dali University,Dali 671003,China
  • Online:2022-12-07 Published:2022-12-07

摘要: 为探究滇西北地区所产车前草的抗氧化活性,本研究分别采用DPPH自由基清除率法、ABTS自由基清除率法以及FRAP总还原能力法对其石油醚提取物、乙酸乙酯提取物、95%乙醇提取物、水提取物的抗氧化活性进行了评价。结果显示,四种提取物均具有一定的抗氧化活性。其中,95%乙醇提取物对DPPH和ABTS自由基的清除效果最好(IC50分别为116.00 mg/L和25.00 mg/L,为Vc的1.2%和5%),水提取物的总抗氧化还原能力最强(浓度为1.00 g/L时,FRAP值为3.42±0.02)。研究表明,滇西北地区所产车前草具有体外抗氧化活性,且高于其他产地,可进一步研究确认其抗氧化机制,为滇西北地区天然抗氧化产物开发提供理论依据。

关键词: 车前草, 滇西北, 体外抗氧化, 自由基清除率, 氧化还原能力

Abstract:

In order to explore the antioxidant activity of plantain grown in northwestern Yunnan,this study used DPPH free radical scavenging rate method,ABTS free radical scavenging rate method and FRAP total reducing ability method to evaluate the antioxidant activity of its petroleum ether extract,ethyl acetate extract,95% ethanol extract and water extract.The results show that the four extracts all have certain antioxidant activity.Among them,95% ethanol extract has the best scavenging effect on DPPH and ABTS free radicals (IC50 is 116.00 mg/L and 25.00 mg/L which was 1.2% and 5% of Vc),and the total reduction ability of water extract is the strongest (when the concentration is 1.00 g/L,the FRAP value is 3.42±0.02).Studies have shown that the plantain grown in northwestern Yunnan has in vitro antioxidant activity,and is higher than that grown in other areas.Further research is needed to confirm its antioxidant mechanism and provide a theoretical basis for the development of natural antioxidant grown in northwestern Yunnan.

Key words: plantain, northwest Yunnan, antioxidant in vitro, free radical scavenging rate, redox capacity

中图分类号:  Q819