天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (10): 1651-1658.doi: 10.16333/j.1001-6880.2023.10.001

• 研究论文 •    下一篇

芪归多糖的分离纯化及体外抑制肿瘤细胞增殖研究

刘文娟1，马善波2，鹿纹菲1，崔东晓1，徐顶巧1，乐世俊1，曹   蔚3，唐于平1*   

  1. 1陕西中医药大学 陕西中医药管理局中药配伍重点研究室，咸阳 712046；2空军军医大学第一附属医院药剂科，西安 710032；
    3西北农林科技大学化学与药学院，杨凌 712100
  • 出版日期:2023-10-28 发布日期:2023-10-31
  • 基金资助:
    陕西省中医药管理局中医药传承创新暨“秦药”开发重点科学研究项目（2021-02-22-007）；国家自然科学基金面上项目（82274084）；陕西省重点研发计划（2022SF-205）

Isolation and purification of Qi-Gui polysaccharides and their in vitro inhibitory activity on tumor cell proliferation

LIU Wen-juan1,MA Shan-bo2,LU Wen-fei1,CUI Dong-xiao1,XU Ding-qiao1,YUE Shi-jun1,CAO Wei3,TANG Yu-ping1*   

  1. 1Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility,Shaanxi University of Chinese Medicine,Xianyang 712046,China;2Department of Pharmacy,Xijing Hospital,Air Force Military Medical University,Xi′an 710032,China;3Shaanxi Key Laboratory of Natural Products & Chemical Biology,School of Chemistry & Pharmacy,Northwest A&F University,Yangling 712100,China
  • Online:2023-10-28 Published:2023-10-31

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摘要：

黄芪-当归配伍为经典补气养血药对，多糖是其重要药效物质。本研究从芪归药对中分离纯化获得6种芪归多糖，CCK-8实验筛选发现其中两种AAPS-1a和AAPS-3a对人结肠癌细胞HCT116和肺癌细胞A549抑制作用显著，对HCT116增殖抑制作用最强，IC50值分别为（47.59±1.3）μg/mL和（18.33±2.5）μg/mL；对A549增值抑制作用次之，IC50值分别为（52.12±1.1）μg/mL和（170.21±2.3）μg/mL。同时，AAPS-1a和AAPS-3a显著诱导HCT116和A549细胞发生凋亡，HCT116细胞凋亡率分别为31.4%和46.5%；A549细胞凋亡率分别为19.3%和33.0%。结构特征分析表明，AAPS-1a和AAPS-3a为分子量分别为7.3×105、8.5×104 Da的均一多糖。本研究从芪归药对中筛选获得具有肿瘤细胞增殖抑制活性的芪归均一多糖，为阐明芪归多糖抗肿瘤结构基础研究提供重要实验依据。

关键词: 黄芪-当归药对, 芪归多糖, 分离纯化, 增殖, 凋亡

Abstract:

Astragali Radix-Angelica Sinensis Radix (Qi-Gui herb pair) has long been used as a classic herb pair for tonifying qi and nourishing blood.Polysaccharide is one of its major active ingredients.Two of the Qi-Gui polysaccharides named AAPS-1a and AAPS-3a,significantly inhibited the proliferation of human colon cancer cells HCT116 and human lung carcinoma cells A549,with IC50 of (47.59±1.3) μg/mL,(18.33±2.5) μg/mL for HCT116 cells,and IC50 of (52.12±1.1) μg/mL,(170.21±2.3) μg/mL for A549 cells.Furthermore,AAPS-1a and AAPS-3a induced apoptosis in HCT116 and A549 cells.The apoptotic rate of the HCT116 cells were 31.4%,46.5% respectively and the apoptotic rate of the A549 cells were 19.3% and 33.0% separately.Structural analysis showed that AAPS-1a and AAPS-3a were homogeneous polysaccharides with molecular weights of 7.3×105 and 8.5×104 Da,respectively.Our study found two homogeneous Qi-Gui polysaccharides with significant anti-tumor activity in vitro,which provide experimental basis for clarifying the anti-tumor structure of polysaccharides in Qi-Gui herb pair.

Key words: Astragali Radix-Angelica Sinensis Radix(Qi-Gui herb pair), Qi-Gui polysaccharides, isolation and purification, proliferation, apoptosis

中图分类号:  R932

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