天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (10): 1775-1781.doi: 10.16333/j.1001-6880.2023.10.014

• 开发研究 • 上一篇    下一篇

光质对等鞭金藻的生长和生物活性产物合成的影响

钟彩荣1,陈钦常1,许涵玥1,曾子航1,徐绍丝1,车丹丹1,2,方静平1*,何勇锦1,2*   

  1. 1福建师范大学生命科学学院;2福建师范大学工业微生物教育部工程研究中心,福州 350117
  • 出版日期:2023-10-28 发布日期:2023-10-31
  • 基金资助:
    国家自然科学基金(41906096);福建师范大学碳中和研究院开放项目(TZH-2022-03);福建省环保科技计划(2023R001)

Effect of different light qualities on the growth and bioactive compounds biosynthesis of Isochrysis sp.ISO-FJ

ZHONG Cai-rong1, CHEN Qin-chang1, XU Han-yue1, ZENG Zi-hang1, XU Shao-si, CHE Dan-dan1,2, FANG Jing-ping1*, HE Yong-jin1,2*   

  1. 1College of Life Sciences, Fujian Normal University;2Engineering Research Center of Industrial Microbiology, Ministryof Education, Fujian Normal University,Fuzhou 350117, China
  • Online:2023-10-28 Published:2023-10-31

摘要: 本实验探究白光(400~700 nm)、红光(620~650 nm)和绿光(500~580 nm)对等鞭金藻(Isochrysis sp.ISO-FJ)的生长和生物活性产物积累的协同影响,以期摸索适宜的光质同时提高其生物活性产物。实验结果表明,等鞭金藻细胞在白光条件下具有最大的生物量。在生物活性产物合成方面,与白光和红光相比,采用绿光培养的等鞭金藻细胞更有利于岩藻黄素、胞外多糖和多不饱和脂肪酸的联产合成。这些结果可表明,等鞭金藻的生长和活性产物合成是受不同光质的调节,为今后定向调控等鞭金藻生长和活性产物合成提供实践依据和理论指导。

关键词: 等鞭金藻, 光质, 岩藻黄素, 胞外多糖, 多不饱和脂肪酸

Abstract:

In order to explore the appropriate light quality to simultaneously improve multiple bioactive compounds productivities, the synergistic effects of white light(400-700 nm), red light(620-650 nm) and green light(500-580 nm) on the microalgal growth and bioactive compounds biosynthesis of Isochrysis sp.ISO-FJ were investigated.The results showed that Isochrysis sp. ISO-FJ gave the highest biomass in the white light.In term of the bioactive compounds, the green light was more beneficial to the coproduction of fucoxanthin, exopolysaccharides and polyunsaturated fatty acids in microalgal cells, in comparison to the white or red light.These results together suggested that the growth and bioactive compounds biosynthesis of Isochrysis sp. were regulated by light qualities, which could offer practical basis and theoretical guidance for the directed regulation of microalgal growth and bioactive compounds by marine microalgae Isochrysis strains.

Key words: Isochrysis sp., light quality, fucoxanthin, exopolysaccharides, polyunsaturated fatty acids

中图分类号:  Q935