This study aimed to investigate the variety and distribution of 7H-azulene［1,2,3-i,j］isoquinoline-7-one (7H-AI7O) components from Acorus traditional Chinese medicine.Using liquid-liquid extraction,solvent extraction,column chromatography separation technology and thin-layer chromatography,the enrichment fragments of 7H-AI7O components from Acori Calami Rhizome and Acori Tatarinowii Rhizome were prepared with neo-tatarine as the reference substance,and then UPLC-Q-TOF/MS² was used to analyze the composition of 7H-AI7O. Identified by thin-layer chromatography,among 10 batches of Acorus traditional Chinese medicine,only batches S2,S4 and S5 of Acori Calami Rhizome and S6 of Acori Tatarinowii Rhizome contained 7H-AI7O components.According to the analysis of the mass spectrometry fragmentation rule of neo-tatarine and target compounds,five 7H-AI7O compounds were detected in batch S2,two 7H-AI7O compounds were detected in batch S4,and only neo-tatarine was detected in batch S5 and S6.Although the number of test samples is limited,according to the analysis of the current results,there are 7H-AI7O components in both Acori Calami Rhizome and Acori Tatarinowii Rhizome,the distribution of the origin of Acori Calami Rhizome containing 7H-AI7O compounds is wider than that of Acori Tatarinowii Rhizome,and types of 7H-AI7O components in Acori Calami Rhizome were more than in Acori Tatarinowii Rhizome.This study provides experimental basis for multiple plant sources,trace component detection,and related mechanisms of action of 7H-AI7O components.