天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (2): 291-301.doi: 10.16333/j.1001-6880.2023.2.013

• 开发研究 • 上一篇    下一篇

盘龙七片中抗炎镇痛生物碱类成分同时测定的方法学研究

何   姬1，刘妍如1*，段金廒2*，唐志书1,3，宋忠兴1，赵艳婷1，张德柱4，杨国伟5   

  1. 1陕西中医药大学 陕西中药资源产业化省部共建协同创新中心，咸阳 712083；2南京中医药大学 江苏省中药资源产业化过程协同创新中心 江苏省方剂高技术研究重点实验室,南京 210023；3中国中医科学院，北京 100700；4陕西盘龙药业集团股份有限公司，商洛 711400；5山西省检验检测中心（山西省标准计量技术研究院），太原 030002
  • 出版日期:2023-02-28 发布日期:2023-03-02
  • 基金资助:
    陕西省科技厅重点产业创新链（群）项目（2020ZDLSF05-08）；国家自然科学基金面上项目（82173970）；陕西省高层次人才特殊支持计划&青年拔尖人才支持计划（2018-33）

Study on the method of simultaneous determination of anti-inflammatory and analgesic alkaloids in Panlongqi tablets

HE Ji1,LIU Yan-ru1 *,DUAN Jin-ao2 *,TANG Zhi-shu1,3,SONG Zhong-xing1,ZHAO Yan-ting1,ZHANG De-zhu4,YANG Guo-wei5   

  1. 1Medicine Shaanxi Provincial and Ministerial Collaborative Innovation Center for Industrialization of Traditional Chinese Medicine Resources,Shaanxi University of Chinese Medicine,Xianyang 712083,China;2 Jiangsu Key Laboratory for High Technology Research of TCM Formulae,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China;3China Academy of Chinese Medical Sciences,Beijing 100700，China; 4Shaanxi Panlong Pharmaceutical Group Co.,Ltd.,Shangluo 711400，China;5Shanxi Provincial Inspection and Testing Center (Shanxi Institute of Standards and Metrology Technology),Taiyuan 030002,China
  • Online:2023-02-28 Published:2023-03-02

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摘要：

采用超高效液相色谱-四极杆-飞行时间质谱联用（UPLC-Q-TOF-MS）技术，对盘龙七片抗炎镇痛生物碱类成分进行表征，共表征出17种生物碱成分；根据表征的结果建立UPLC-ESI-MS/MS定量分析方法以用于盘龙七片质量分析评价，结果表明17种生物碱在各自浓度范围内线性关系良好，r≥0.9 990，仪器精密度、方法重复性、供试品稳定性均良好，平均加样回收率为86.42%~112.75%，RSD为1.49%~5.59%（n= 6）。本研究所建立的方法准确、重复性良好，可用于盘龙七片中17种生物碱的定量测定，为完善盘龙七片质量控制的潜在新指标提供了参考。

关键词: 盘龙七片, 生物碱, 成分分析, UPLC-Q-TOF-MS/MS, UPLC-ESI-MS/MS

Abstract:

The ultra performance liquid chromatography tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) technology was utilized to identify the anti-inflammatory and analgesic alkaloids components of Panlongqi tablets,and a total of seventeen alkaloids components were characterized;based on the characterization results,UPLC-ESI-MS/MS quantitative analysis method was established for the quality analysis of Panlongqi tablets.The results showed that seventeen alkaloids had good linear relationship in their respective concentration ranges,r≥0.9990,the average recoveries rate was 86.42%-112.75%,the RSDs of 1.49%-5.59% (n=6).The method established in this study is accurate and reproducible for the quantitative determination of seventeen alkaloids in Panlongqi tablets,which provides a reference for the improvement of potential new indicators for quality control of Panlongqi tablets.

Key words: Panlongqi tablets, alkaloids, composition analysis, UPLC-Q-TOF-MS/MS, UPLC-ESI-MS/MS

中图分类号:  R286.0 R917

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