金露梅中一种α-葡萄糖苷酶抑制剂的提取富集及活性研究

doi:10.16333/j.1001-6880.2023.3.012

天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (3): 460-466.doi: 10.16333/j.1001-6880.2023.3.012

金露梅中一种α-葡萄糖苷酶抑制剂的提取富集及活性研究

张俊焱1，李成慧1，左文明1，李锦萍1，刘力宽1*，曾阳2*

1青海师范大学：2高原科学与可持续发展研究院，西宁 810008

出版日期:2023-03-28 发布日期:2023-03-28
基金资助:
青海省科技国际合作专项（2022-HZ-815）；青海省科技创新“药用植物资源综合利用与开发研究”创新团队（2020-2023）；青海省高端创新人才千人计划（2017年）；青海省青藏高原药用动植物资源重点实验室（2020-ZJ-Y40）

Extraction,enrichment and activity of an α-glucosidase inhibitor from Potentilla fruticosa L.

ZHANG Jun-yan1,LI Cheng-hui1,ZUO Wen-ming1,LI Jin-ping1,LIU Li-kuan1*,ZENG Yang2*

1Qinghai Normal University;2Academy of Plateau Science and Sustainability,Xining 810008,China

Online:2023-03-28 Published:2023-03-28

摘要/Abstract

摘要： 本研究采用AB-8大孔吸附树脂、半制备高效液相色谱对金露梅枝叶中槲皮素-7-O-β-D-葡糖苷酸提取分离，并通过¹³C NMR鉴定其化学结构，最后采用以4-硝基酚-α-D-吡喃葡萄糖苷（pNPG）为底物的酶抑制剂筛选模型检测其对α-葡萄糖苷酶的抑制活性和类型。结果显示：槲皮素-7-O-β-D-葡糖苷酸分布在20%乙醇大孔吸附树脂（AB-8）洗脱液中，采用半制备高效液相色谱和高效液相色谱分离纯化得槲皮素-7-O-β-D-葡糖苷酸2481.8 mg，纯度为97.95%。活性检测结果表明，槲皮素-7-O-β-D-葡糖苷酸对α-葡萄糖苷酶的最大抑制率为94.67%，IC₅₀为0.259 mmol/L，抑制率显著高于阳性对照药物阿卡波糖，且抑制剂类型为竞争性抑制。

关键词: 金露梅, 槲皮素-7-O-β-D-葡糖苷酸, 半制备高效液相色谱, 提取富集, α-葡萄糖苷酶抑制剂

Abstract:

The quercetin-7-O-β-D-glucuronic acid compound in the branches and leaves of Potentilla fruticosa L. was extracted and separated,and its chemical structure was identified by ¹³C NMR.It was prepared by AB-8 macroporous adsorption resin and semi-preparative high-performance liquid chromatography. Purification and enrichment.Finally,an enzyme inhibitor screening model with 4-nitrophenol-α-D-glucopyranoside (pNPG) as a substrate is used to detect the activity of the target compound and the type of inhibitor.The results showed that the ethanol of 20% macroporous adsorption resin (AB-8) contained quercetin-7-O-β-D-glucuronide.After preparative high-performance liquid chromatography and high-performance liquid chromatography,the quercetin -7-O-β-D-glucuronide was obtained at 2481.8mg,purity 97.95%.The activity test results showed that the maximum inhibitory rate of quercetin-7-O-β-D-glucuronide against α-glucosidase was 94.6 7%,IC₅₀ was 0.259 mmol/L,and the inhibition rate was significantly higher than of the positive control drug acarbose,and the inhibitor type was competitive inhibition.

Key words: Potentilla fruticosa L., quercetin-7-O-β-D-glucuronide, semi-preparative HPLC, extraction and enrichment, α-glucosidase inhibitor

中图分类号: R284 R285

张俊焱, 李成慧, 左文明, 李锦萍, 刘力宽, 曾阳. 金露梅中一种α-葡萄糖苷酶抑制剂的提取富集及活性研究[J]. 天然产物研究与开发, 2023, 35(3): 460-466.

ZHANG Jun-yan, LI Cheng-hui, ZUO Wen-ming, LI Jin-ping, LIU Li-kuan, ZENG Yang. Extraction,enrichment and activity of an α-glucosidase inhibitor from Potentilla fruticosa L.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(3): 460-466.

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金露梅中一种α-葡萄糖苷酶抑制剂的提取富集及活性研究

Extraction,enrichment and activity of an α-glucosidase inhibitor from Potentilla fruticosa L.

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