天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (增刊1): 73-79.

• 开发研究 • 上一篇    下一篇

响应面优化提取山莨菪生物碱的工艺及其抗炎活性研究

丁   金1,2，铁芳芳1，胡   娜1，王洪伦1，董   琦1*   

  1. 1中国科学院西北高原生物研究所 中国科学院藏药研究重点实验室 青海省藏药研究重点实验室，西宁 810008；2中国科学院大学，北京 100049
  • 出版日期:2023-06-28 发布日期:2023-06-13
  • 基金资助:
    中国科学院青海省人民政府三江源国家公园联合研究专项（LHZX-2020-09）

Study on optimization of the extraction process of total alkaloids in Anisodus tanguticus (Maxim.) Pascher by response surface methodology and its anti-inflammatory activity

DING Jin1,2,TIE Fang-fang1,HU Na1,WANG Hong-lun1,DONG Qi1*   

  1. 1Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology Qinghai Provincial & Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;2University of Chinese Academy Sciences,Beijing 100049,China
  • Online:2023-06-28 Published:2023-06-13

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摘要：

以山莨菪（Anisodus tanguticus (Maxim.) Pascher）的根为实验材料，以总生物碱的含量为检测指标，应用响应面法对山莨菪根中总生物碱的提取工艺进行了优化。结果显示，当提取条件为液料比61 mL/g，乙醇浓度78%，温度80 ℃，提取时间2 h，提取2次，山莨菪根提取物中总生物碱的理论含量值可达1.687%，总生物碱含量达到最高值。按照最佳提取工艺条件提取山莨菪根、种子和地上部分的总生物碱，总生物碱的含量分别为（1.639 ± 0.033）%、（0.950 ± 0.019）%、（0.852 ± 0.031）%。通过检测山莨菪生物碱对脂多糖（LPS）诱导小鼠腹腔巨噬细胞（RAW 264.7）的一氧化氮（NO）的影响，发现20~80 μg/mL的根部和地上部分的生物提取物能够显著抑制RAW 264.7细胞内NO的产生，并呈剂量依赖效应。本研究为山莨菪药材中生物碱提取分离的工业化生产提供理论指导，为明确山莨菪抗炎作用提供了数据支撑，为山莨菪的开发与利用提供了依据。

关键词: 山莨菪, 总生物碱, 提取工艺, 响应面法, 抗炎

Abstract:

In this study,the root of Anisodus tanguticus (Maxim.) Pascher was used as the experimental material and the content of total alkaloids was considered as the detection index.The optimum extraction conditions of total alkaloids in A. tanguticus roots were investigated using response surface methodology (RSM).The optimum extraction conditions were:liquid-solid ratio of 61∶1,ethanol concentration of 78%，extraction temperature of 80 ℃,extraction times of 2,and extraction time of 2 h,with a theoretical total alkaloid content of 1.687%.According to the optimal extraction process conditions,the total alkaloids from the roots,seeds and above-ground parts of Anisodus henryi were extracted,and the total alkaloid contents were (1.639 ± 0.033)%,(0.950 ± 0.019)% and (0.852 ± 0.031)%,respectively.By detecting the effect of anisodus alkaloids on lipopolysaccharide (LPS)-induced nitric oxide (NO) in mouse peritoneal macrophages (RAW 264.7),biological extracts of 20-80 μg/mL roots and arcialL parts were found it can significantly inhibit the production of NO in RAW 264.7 cells with a dose-dependent effect.This study provides theoretical guidance for the industrial production of the extraction and separation of alkaloids from A. tanguticus  medicinal materials,provides data support for clarifying the anti-inflammatory effects of A. tanguticus,and provides a basis for the development and utilization of A. tanguticus.

Key words: Anisodus tanguticus (Maxim.) Pascher, total alkaloids, extraction, response surface methodology, anti-inflammatory

中图分类号:  R285.5

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