天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (增刊2): 99-103.

• 开发研究 • 上一篇    下一篇

金银花不同提取方法的比较研究

李欣聪1,宋学英2,施子涵1,王彩芳1*   

  1. 1首都医科大学药学院;2首都医科大学中心实验室,北京 100069
  • 出版日期:2023-12-27 发布日期:2023-12-11
  • 基金资助:
    首都医科大学本科生科研创新项目(XSKY2020041)

A comparative study on different extract methods of Lonicera japonica

LI Xin-cong1,SONG Xue-ying2,SHI Zi-han1,WANG Cai-fang1*   

  1. 1School of Pharmaceutical Sciences,Capital Medical University;2Central Laboratory,Capital Medical University,Beijing 100069, China
  • Online:2023-12-27 Published:2023-12-11

摘要: 本文采用超声法(A法)和组织破碎法(B法)两种方法提取中药金银花中的有效成分,以绿原酸、芦丁和木犀草苷3种活性成分作为评价指标,通过高效液相色谱法进行含量测定,比较两种提取方法对金银花药材3种活性成分提取率的影响。结果表明两种提取方法所得3种指标性成分的提取率有一定差异,尤其采用B法提取所得绿原酸含量远高于A法。另外B法中提取时间大大缩短,这很大程度上提高了提取效率,以上结果可以为金银花提取物的生产提供一定参考。

关键词: 金银花, 组织破碎法, 超声提取法, HPLC

Abstract:

In this paper,ultrasonic method (method A) and tissue crushing method (method B) were used to extract the active components of Lonicera japonica.Chlorogenic acid,rutin and luteoloside were used as evaluation indexes,and the effects of two extraction methods on the extraction rates of the three active components of L. japonica were compared by HPLC.The results showed that there were some differences in the extraction rates of the three index components obtained by metheod A and B,especially the content of chlorogenic acid extracted by method B was far higher than that extracted by method A.In addition,compared with the traditional method A,the extraction time of method B is greatly shortened,which greatly improves the extraction efficiency.The results can provide a certain reference for the production of honeysuckle extract.

Key words: Lonicera japonica, tissue fragmentation, ultrasonic extraction method, HPLC

中图分类号:  R284