天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (1): 72-77.doi: 10.16333/j.1001-6880.2024.1.008

• 研究简报 • 上一篇    下一篇

尖尾芋石油醚部位化学成分及其乳腺癌细胞毒活性研究

王   鹏1，陈   娅1，彭兰淳1，郑青竹1，彭江丽1,2，彭求贤1,3*   

  1. 1湖南中医药大学药学院；2湖南省中药活性物质筛选工程技术研究中心；3湘产大宗药材品质评价湖南省重点实验室，长沙410208 
  • 出版日期:2024-01-23 发布日期:2024-01-23
  • 基金资助:
    国家自然科学基金（81973593）；湖南省自然科学基金-科药联合项目（2022JJ80087）；湖南中医药大学研究生广东一方制药有限公司科研基金（2021YF01）；湖南中医药大学中药学一流学科基金（校行科字（2018）3号）

Study on thechemical constituents from the petroleum ether extract of Alocasia cucullata (Lour.) Schott and their breast cancer cell cytotoxicity

WANG Peng1,CHEN Ya1,PENG Lan-chun1,ZHENG Qing-zhu1,PENG Jiang-li1, 2,PENG Qiu-xian1,3*   

  1. 1School of Pharmacy,Hunan University of Chinese Medicine; 2Hunan Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine;3Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,Hunan University of Chinese Medicine,Changsha 410208,China
  • Online:2024-01-23 Published:2024-01-23

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摘要：

研究天南星科海芋属植物尖尾芋 Alocasia cucullata (Lour.) Schott石油醚部位化学成分。采用硅胶、Sephadex LH-20等色谱方法进行分离纯化，运用波谱学技术进行结构鉴定。采用CCK-8法测试化合物对人乳腺癌MDA-MB-231、MCF-7细胞及小鼠乳腺癌4T1细胞的体外细胞毒活性。从尖尾芋根茎石油醚部位中分离得到15个化合物，分别为对羟基苯甲醛（1）、苯甲酸（2）、香草酸乙酯（3）、没食子酸乙酯（4）、7-羟基香豆素（5）、肉豆蔻酸甲酯（6）、角鲨烯（7）、齐墩果酸（8）、熊果酸（9）、香紫苏醇（10）、香紫苏内酯（11）、原儿茶醛（12）、原儿茶酸（13）、二十八烷醇（14）和没食子酸（15），化合物2~15为海芋属首次分离得到，所有化合物均为尖尾芋首次分离得到。乳腺癌细胞毒活性研究显示：化合物4、8和9对3种乳腺癌细胞均有一定的抑制作用。

关键词: 尖尾芋, 化学成分, 结构鉴定, 乳腺癌, 细胞毒活性

Abstract:

To study the chemical constituents of the petroleum ether extract from Alocasia cucullata (Lour.) Schott,a plant species belonging to the Araceae family.Silica gel,Sephadex LH-20 and other chromatographic methods were used for separation and purification,and spectroscopic techniques were employed for structural identification.The in vitro assessment of all isolated compounds was conducted using the CCK-8 assay on human breast cancer cell lines MDA-MB-231 and MCF-7,as well as mouse breast cancer cells 4T1.Fifteen compounds were isolated from the petroleum ether fraction of the rhizome of A. cucullata and their structures were identified as p-hydroxybenzaldehyde (1),benzoic acid (2),ethyl vanillate (3),ethyl gallate (4),7-hydroxycoumarin (5),myristate (6),squalene (7),oleanolic acid (8),ursolic acid (9),sclareol (10),sclareolide (11),protocatechualdehyde (12),protocatechuic acid (13),octacosanol (14) and gallic acid (15).Compounds 2-15 were first isolated from genus Alocasia.All the compounds were first isolated from A. cucullata.The cytotoxicity study of breast cancer cell showed that compounds 4,8 and 9 had inhibitory effect on all three kinds of breast cancer cell lines.

Key words: Alocasia cucullata (Lour.) Schott, chemical composition, structure identification, breast cancer, cell cytotoxicity

中图分类号:  R932

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