天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (2): 293-302.doi: 10.16333/j.1001-6880.2024.2.012

• 开发研究 • 上一篇    下一篇

骨碎补UPLC指纹图谱与人骨肉瘤细胞MG63中碱性磷酸酶活性的谱效关系研究

陈香丽，张慧敏，张紫佳*   

  1. 上海中医药大学中药研究所 中药标准化教育部重点实验室，上海 201203
  • 出版日期:2024-02-23 发布日期:2024-02-23
  • 基金资助:
    国家重点研发计划子任务（2019YFC1711004）

Study on the spectrum-effect relationship between UPLC fingerprint and alkaline phosphatase activity in osteosarcoma MG63 cell of Drynariae Rhizoma

CHEN Xiang-li,ZHANG Hui-min,ZHANG Zi-jia*   

  1. The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
  • Online:2024-02-23 Published:2024-02-23

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摘要： 建立骨碎补生品和砂烫品提取物的UPLC指纹图谱，并研究骨碎补提取物与人骨肉瘤细胞MG63中碱性磷酸酶（ALP）活性之间的谱效关系。采用UPLC法建立骨碎补生品和烫品的指纹图谱并通过UPLC-Q-TOF MS法对其化学成分进行鉴别；通过测定MG63细胞中ALP活性检测骨碎补提取物对MG63细胞成骨分化的影响；采用灰色关联度分析、双变量相关性分析以及偏最小二乘回归分析法分析骨碎补生品和烫品的UPLC特征指纹峰与其促ALP活性之间的谱效关系。在标定的12个共有峰中，确定了4、5、12号峰与ALP活性呈正相关，其对应的物质依次是咖啡酸-4-O-β-D-吡喃葡萄糖苷、儿茶素7-葡糖苷、柚皮苷，为深入研究骨碎补的促成骨分化活性物质基础提供依据。

关键词: 骨碎补, UPLC指纹图谱, 碱性磷酸酶活性, 谱效关系, 人骨肉瘤细胞MG63

Abstract:

To establish a UPLC fingerprint of the extract of Drynariae Rhizoma before and after processing,and to study the spectrum-effect relationship between the extract of Drynariae Rhizoma and alkaline phosphatase (ALP) activity in osteosarcoma MG63 cell line.Using UPLC method to establish the fingerprint of raw materials and processing slices of Drynariae Rhizoma and identified their chemical components by UPLC-Q-TOF MS;The ALP activity was measured to determine the effect of Drynariae Rhizoma extract on osteosarcoma MG63 cell line;Grey correlation analysis,bivariate correlation analysis,and partial least square regression analysis were used to analyze the spectrum-effect relationship between the UPLC characteristic fingerprint peaks and ALP activity of the raw and processed products of Drynariae Rhizoma.Among the 12 characteristic fingerprint peaks calibrated,it was determined that peaks 4,5 and 12 were positively correlated with ALP activity,and the corresponding substances were caffeic acid 4-O-β-D-glucopyranoside,catechin 7-glucoside and naringin.It provides a reference for in-depth research on the active material basis of Drynariae Rhizoma in promoting osteogenic differentiation effect.

Key words: Drynariae Rhizoma, UPLC fingerprint, alkaline phosphatase activity, spectrum-effect relationship, osteosarcoma MG63 cell line

中图分类号:  R284.1

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