关键词: 栀子, 栀子苷, 绿原酸, 响应曲面, 提取工艺

Abstract: To determine gardenoside and chlorogenic acid in Gardenia jasminoides Ellis,response surface methodology (RSM) was designed to optimize the extraction method based on the single-factor experiments.A second order quadratic mathematical model for the extraction of gardenoside and chlorogenic acid from Gardenia jasminoides Ellis was established.Its validity was verified,and the effects of solid-liquid ratio,ethanol concentration,ultrasonic time on the extraction yield were investigated.The optimal extraction conditions were determined as follows:liquid/solid ratio of 42.67 mL/g,methanol concentration of 56.89%,ultrasonic time of 15.67 min.Under theese conditions,the yield of chlorogenic acid and gardenoside was 27.38 mg/g,48.47 mg/g,with only 0.60%,1.16% differences from the predictive values of RSM.The optimized extraction process was suitable and feasible.

Key words: Gardenia jasminoides Ellis, gardenoside, chlorogenic acid, response surface methodology, extraction