关键词: 芪胶升白胶囊, CTX, S₁₈₀, 增效, 减毒

Abstract: This study was aimed to explore synergistic and attenuation effect of Qijaoshengbai capsule(QSC)in combination with cyclophosphamide(CTX)on S₁₈₀ tumor-burdened mice.S₁₈₀ tumor cells was inoculated subcutaneously into the right armpit of mice to induce S₁₈₀ tumor bearing mice model,then the models were randomly divided into five groups,consisting of model group,CTX(30 mg/kg)group,CTX and QSC low,medium and high-dose(0.5,1.0,2.0 g/kg)groups,with twelve mice in each groups.Tumor volume and body weight were measured respectively for 15 consecutive drug administration days.The blood cell count,visceral index and tumor inhibition ratio were calculated after 15 drug administration days.HE staining was carried out to observe the changes of tumor tissue morphology,and the apoptosis of tumor cells was detected by Annexin V/PI flow cytometer method.The results showed that compared with CTX group,the tumor volume in each doses of QSC in combination with CTX groups were decreased significantly,both tumor inhibition rate and apoptosis were increased in combination groups,and the thymus index and spleen index were also increased.The high dose of QSC in combination with CTX group increased WBC count significantly(P<0.01) and its tumor tissue necrosis obviously examined under a microscope.Based these result,it was concluded that QSC in combination with CTX had synergistic effect on S₁₈₀ tumor-burdened mice.

Key words: Qijiaoshengbai capusle, cyclophosphamide, S₁₈₀, synergistic and attenuation effect