关键词: 蜘蛛香, 化学成分, 波谱特征

Abstract: Ten compounds were isolated from the root of Valeriana jatamansi Jones by ODS column,silica gel column and Sephadex LH-20 chromatography, etc. Their structures were elucidated by NMR,and were identified as double-tetrahydrofuran lignans-4-O-β-D-Glucopyranoside(1),4-hydroxybenzyl-β-D-glucopyranoside(2),methylarbutin(3),4-［(E)-3-ethoxyprop-1-enyl］-2-methoxyphenol(4),eugenyl-O-D-glunopyranoside(5),ethylconiferin(6),8-hydroxypinoresinol-4′-O-β-D-glucopyranoside(7), palmitic acid(8),protocatechuic acid(9),3-(4-β-D-Glucopyranosyloxy-3,5-dimethoxy)-phenyl-2E-propenol(10).Compounds 5、6 were isolated from Valerianaceae for the first time.The rule of hydroxylation and glycosidation shift effects on bisepoxy lignans were obtained by comparing the information of ¹³C NMR with known analogous.Glycosidation and hydroxylation ¹³C NMR chemical shift effects on double-tetrahydrofuran lignans were closely related to the number of hydroxyl-substitution.The rules for effects of glycosidation at C-1′and ortho-C-3′ downshift about △δ1.7 in no hydroxy or mono-hydroxy substitution,C-1′downshift about △δ2.4 in double-hydroxy substitution,while ortho-C-3′ only changes a little(△δ0.5).In hydroxylation,tertiary carbon downshift to quaternary carbon(△δ34.5-37.5) when hydroxy substitution at C-8 or C-8′.Besides,the C-1 upshift about △δ3.9-5.9 because of the effect of distance effect.C-8′ on mono-hydroxy substituted aromatic ring moves to low field(△δ6.0),while downshifts △δ34.5 for double-hydroxy substitution.The rules of displacement provided suggestion for the confirmation of double-tetrahydrofuran lignans,especially the position of hydroxylation and glycosylation.

Key words: Valeriana jatamansi Jones, chemical constituent, spectral characteristics