关键词: 黑果腺肋花楸花色苷, 皮肤成纤维细胞, 辐射损伤, 细胞内活性氧

Abstract: In order to study the protective effect of Aronia melanocarpa anthocaynins against UV-induced damages in human skin fibroblast,the cells were divided into control group,UVA radiation group and anthocyanidin group.The protective effects of different concentrations of anthocyanins on cell proliferation were investigated using MTT method.The reactive oxygen species(ROS)was evaluated by fluorescence and chemiluminescence,the MMP-1 protein in cells were detected by ELISA assay.The MTT analysis result showed that 125 μg/mL of anthocyanin had an excellent protective effect on cell proliferation.The contents of ROS and MMP-1 in 125 μg/mL anthocaynin group were significant lower than those in UVA radiation group(P<0.01).Hence,it was concluded that A.melanocarpa anthocaynins was able to reduce UV-induced oxidative damage and played an anti-UV radiation role in human skin fibroblast.

Key words: Aronia melanocarpa anthocaynins, fibroblasts, radiation damage, reactive oxygen species