天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (8): 1192-1198.doi: 10.16333/j.1001-6880.2016.8.004

• 研究论文 • 上一篇    下一篇

不同种质和地域太子参氨基酸柱前衍生HPLC测定及多元统计分析

马阳,侯娅,邹立思,刘训红*,罗益远,刘娟秀   

  1. 南京中医药大学药学院,南京 210023
  • 出版日期:2016-08-30 发布日期:2017-01-17

Determination and Multivariate Statistical Analysis of Amino Acids in Pseudostellariae Radix from Different Idioplasm and Cultivation Regions by HPLC with Pre-column Derivatization

MA Yang,HOU Ya,ZOU Li-si,LIU Xun-hong*,LUO Yi-yuan,LIU Juan-xiu   

  1. Nanjing University of Chinese Medicine,Nanjing 210023,China
  • Online:2016-08-30 Published:2017-01-17

摘要: 探讨种质和生态环境因素对太子参中氨基酸类成分积累的影响。采用柱前衍生化高效液相色谱(HPLC)测定太子参中20种游离氨基酸含量,并进行多元统计分析。排序分析表明同一种质不同地域、同一地域不同种质太子参间均存在一定差异;主成分分析显示谷氨酸、苏氨酸、蛋氨酸、异亮氨酸、脯氨酸、γ-氨基丁酸为太子参特征氨基酸,综合评价表明就氨基酸而言江苏句容栽培的ZS2太子参品质较好;单因素方差分析表明栽培地域与其特征氨基酸存在显著相关。生态环境因子是影响太子参中氨基酸含量的主要因素。本研究为深入研究太子参药材的品质形成机制提供基础资料。

关键词: 太子参, 种质和地域, 高效液相色谱, 氨基酸, 多元统计分析

Abstract: To investigate the influence of geographical and germplasm factors on amino acid composition and content in Pseudostellariae Radix.The content of 20 amino acids were determined by HPLC with pre-column derivatization and for the multivariate statistical analysis.Sequencing analysis showed that there were differences in Pseudostellariae Radix between the same germplasm of different regions and the same region of the different germplasm.The principal components analysis showed that glutamic acid,threonine,methionine,isoleucine,proline and γ-aminobutyric acid were characteristic amino acids in Pseudostellariae Radix.Comprehensive evaluation showed that ZS2 cultivated in Jiangsu Jurong had the better quality in terms of amino acids.Oneway ANOVA showed there was significant correlation between region and the characteristic amino acids.The region was the main factor affecting the differences of amino acids in Pseudostellariae Radix.This study provided a scientific basis for the investigation of the influence of the quality formation mechanism of Pseudostellariae Radix.

Key words: Pseudostellariae Radix, idioplasm and cultivation regions, HPLC, amino acids, multivariate statistical analysis

中图分类号: 

R932