天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (8): 1256-1261.doi: 10.16333/j.1001-6880.2016.8.015

• 研究简报 • 上一篇    下一篇

野生蓝莓抑菌活性物质的分离及对金黄色葡萄球菌的抑制作用

魏彩虹1,孙晓红1*,赵勇1,吴启华2,潘迎捷1   

  1. 1 上海海洋大学食品学院 上海水产品加工及贮藏工程技术研究中心 农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海 201306;2 美国USDA-ARS研究中心,美国加利福尼亚州 94710
  • 出版日期:2016-08-30 发布日期:2017-01-17

Separation and Antibacterial Effects of Chinese Wild Blueberry’s Fractional Components

WEI Cai-hong1,SUN Xiao-hong1*,ZHAO Yong1,Vivian C.H.Wu2,PAN Ying-jie1   

  1. 1Collage of Food Science and Technology,Shanghai Ocean University,Shanghai Engineering Research Center  of AquaticProduct Processing & Preservation,Laboratory of Quality & Safety Risk Assessment for Aquatic  Products on Storage and Preservation (Shanghai),Ministry of Agriculture,Shanghai 201306,China;  2Produce Safety and Microbiology Research Unit,USDAARS,CA 94710,USA
  • Online:2016-08-30 Published:2017-01-17

摘要: 本文采用甲醇:水:甲酸(85:15:0.5)溶剂提取中国野生蓝莓的活性成分,并用C-18反相柱对粗提物进行分离,得到糖酸混合物、酚类物质和花青素类物质三种分离物。分别用GB/T 5009.7-2008法、Folin-Ciocalte法、pH示差法测量分离物中的总糖、总酚和花青素含量,并通过二倍稀释法研究了各分离物对金黄色葡萄球菌(AB91093、ATCC26001)的抑制作用及对细菌细胞膜完整性的影响。研究结果表明:蓝莓粗提物中的总糖、总酚与花青素类物质含量分别为4.76%、7.88 mg/g、124.45 mg/100 g,通过C-18反相柱所获产物的分离效果较好。其中,花青素类物质为蓝莓粗提物抑菌作用的主要活性成分,对金黄色葡萄球菌的MIC值和MBC值分别为0.21 mg/mL与0.26 mg/mL;同时,对细胞膜完整性的破坏效果与抑菌活性一致,依次为蓝莓粗提物>花青素类物质>酚类物质>糖酸混合物。

关键词: 野生蓝莓, 糖, 酚, 花青素, 金黄色葡萄球菌

Abstract: The antibacterial effects of Chinese wild blueberry extracts and its different fractions on Staphyloccocus aureus (AB91093,ATCC26001) in vitro were investigated.Methanol:water:formic acid (85:15:0.5) was used to extract Chinese wild blueberry,and the extract was separated using C-18 Sep-Pak cartridge to afford mixture of organic acids and sugar,phenolics and anthocyanins.The content of total sugar,phenolics and anthocyanins in the fractions were determined and their antibacterial effect against S.aureus were investigated respectively.Moreover,their influence on bacteria cell membrane integrity was studied.The contents of total sugar,phenolics and anthocyanins in the extract were 4.76%,7.88 mg/g and 124.45 mg/100 g,respectively.Anthocyanins showed the highest antibacterial effects among blueberry extracts,its MIC and MBC were 0.21 mg/mL and 0.26 mg/mL,respectively.The damage of S.aureus cell membrane integrity was the same as their antibacterial effect.Blueberry extract was the best followed by anthocyanins,the mixture of organic acids and sugar.

Key words: wild blueberry, sugar, phenolics, anthocyanins, Staphyloccocus aureus

中图分类号: 

R285