化瘀通络中药对糖尿病肾病瘀血阻络证大鼠肾皮质核因子κB表达的影响

doi:10.16333/j.1001-6880.2017.1.004

摘要/Abstract

摘要： 探讨化瘀通络中药对糖尿病肾病（DN）瘀血阻络证大鼠肾皮质核因子-κB表达的影响。60只大鼠选取10只为正常组，余大鼠予高脂饲料喂养联合小剂量链脲佐菌素腹腔注射建立糖尿病模型，成模大鼠随机分为模型组、厄贝沙坦组及中药组，相应药物剂量灌胃，16周末测量各组大鼠饮水量、饮食量、尿量、体重及空腹血糖。检测不同时间点（第3天、第8周、第16周）各组大鼠24 h尿蛋白定量（24 hUTP）的变化，留取肾皮质观察肾脏病理损伤及采用Real-time PCR、Western-blot检测NF-κB p65 mRNA及蛋白的表达。结果显示与正常组比较，模型组大鼠出现明显多饮、多食、多尿、消瘦的症状（P<0.05），第8、16周24 hUTP明显增多（P<0.05），NF-κB p65 mRNA及蛋白的表达增强（P<0.05），肾脏病理出现明显损伤；与模型组比较，两干预组在第8周、16周24 hUTP明显减少（P<0.05），NF-κB p65 mRNA及蛋白的表达明显下调（P<0.05），中药组饮水量、尿量、体重有显著改善（P<0.05）；与厄贝沙坦组比较，中药组饮水量、第16周24 hUTP及NF-κB p65蛋白表达明显减少（P<0.05）。结果表明化瘀通络中药抑制NF-κB p65的高表达可能是该药物对DN大鼠肾脏保护作用的靶点之一。

关键词: 糖尿病肾病, 化瘀通络中药, NF-&kappa, B, 蛋白尿, 炎症

Abstract: To explore the intervention effect of Huayu Tongluo Traditional Chinese Medicine on nuclear factor kappa B in renal cortex of blood stasis syndrome rats with diabetic nephropathy (DN).Ten rats were selected as normal group from sixty rats,the models were established by high-fat diet combined with low-dose streptozotocin intraperitoneal injection,and then were randomly divided into model group,irbesartan treatment group and Chinese medicine group after successful modeling.Rats in each treatment group were fed with corresponding drug intragastrically.At the end of the 16th week,water intake,food intake,urine output,body weight and fasting blood glucose were detected.24 h urinary total protein (24 hUTP) of rats were measured at different time point (3rd day,8th week and 16th week) in each group.Renal cortex were conserved to observe the pathological injury and the mRNA and protein expression of NF-κB p65 in renal cortex were detected by Real-time PCR,Western-blot.The results showed that compared with normal group,model group rats appeared obvious polydipsia,polyphagia,polyuria,emaciation (P<0.05),8th and 16th week of 24 hUTP increased significantly (P<0.05),the mRNA and protein expression of NFκB p65 enhanced (P<0.05).Renal pathology appeared obvious injury.Compared with model group,24 hUTP of two intervention groupsdecreased significantly at 8th week and 16th week (P<0.05),the mRNA and protein expression of NFκB p65 decreased significantly (P<0.05),water intake,urine output,body weight of rats in Chinese medicine group improved significantly (P<0.05).Compared with irbesartan group,water intake,24 hUTP at 16th week of the Chinese medicine group and the protein expression of NF-κB p65 decreased significantly (P<0.05).The results showed that inhibiting the high expression of NF-κB p65 can be one of the targets of the medicine on the protective effect of the kidney of DN rats.

Key words: diabetic nephropathy, Huayu Tongluo Traditional Chinese Medicine, NF-&kappa, B, proteinuria, inflammatory

中图分类号:

R255.4

白璐1，霍贝贝1，陈志强1,2*，方敬3，郭倩1. 化瘀通络中药对糖尿病肾病瘀血阻络证大鼠肾皮质核因子κB表达的影响[J]. 天然产物研究与开发, 2017, 29(1): 22-26.

BAI Lu1,HUO Bei-bei1,CHEN Zhi-qiang1,2*,FANG Jing3,GUO Qian1. Effect of Huayu Tongluo Traditional Chinese Medicine on Nuclear Factor kappa B in Renal Cortex of Blood Stasis Syndrome Rats with Diabetic Nephropathy[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(1): 22-26.

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化瘀通络中药对糖尿病肾病瘀血阻络证大鼠肾皮质核因子κB表达的影响

Effect of Huayu Tongluo Traditional Chinese Medicine on Nuclear Factor kappa B in Renal Cortex of Blood Stasis Syndrome Rats with Diabetic Nephropathy

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