天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (1): 106-109.doi: 10.16333/j.1001-6880.2017.1.020

• 开发研究 • 上一篇    下一篇

中药矮地茶不同洗脱部位抗病毒活性研究

刘相文,侯林,张晓平,王剑,田景振*   

  1. 山东中医药大学药学院,济南 250355
  • 出版日期:2017-01-31 发布日期:2017-02-17

Antiviral Activity of Different Parts of Ardisia japonica (Thunb.)Blume

LIU Xiang-wen,HOU Lin,ZHANG Xiao-ping,WANG Jian,TIAN Jing-zhen*   

  1. College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China
  • Online:2017-01-31 Published:2017-02-17

摘要: 采用细胞体外抗病毒实验CPE法结合CCK-8试剂盒,以TI值(治疗指数)为研究指标,研究矮地茶及其不同洗脱部位对呼吸道合胞病毒(RSV)、单纯疱疹病毒(HSV-1)、柯萨奇病毒(COX-B5)和手足口病毒(EV71)的抑制作用。结果表明矮地茶对RSV、HSV-1、COX-B5有显著杀灭作用。70%乙醇部位对COX-B5直接杀灭效果较好,TI值为16.709,较阳性对照药利巴韦林(TI值为17.482)作用效果相差不大。说明矮地茶提取物具有抗RSV、HSV-1、COX-B5的活性。

关键词: 矮地茶, 抗病毒, RSV, HSV-1, COX-B5

Abstract: In this study,the invitro antiviral activity of Ardisia japonica (Thunb.)Blume were evaluated by cytopathic effect (CPE) method and CCK-8 kit with 4different strains of viruses,including RSV,HSV-1,COX-B5 and EV71.The therapeutic indexes were calculated by Reed-Muench method.Antivirus stability experiments of A.jonica were carried out at the same time.The results showed thecytopathic effect caused byRSV,HSV-1 and COX-B5were significantly inhibited,the TI of 70% ethanol parts and ribavirin were 16.709 and 17.482,respectively.It showed that 70% ethanol parts was as effective as the ribavirin.All the results indicated that A.japonica had antiviral activity against RSV,HSV-1 and COX-B5.

Key words: Ardisia japonica (Thunb.)Blume, antiviral, RSV, HSV-1, COX-B5

中图分类号: 

R285.5