天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (Suppl1): 137-139.doi: 10.16333/j.10016880.2016.S.031

• 开发研究 • 上一篇    下一篇

棕榈粕甘露聚糖的制备与分析

王韧1,倪娇娇1,盛义保2,曹树青1,刘国庆2*,樊婷婷1*   

  1. 1合肥工业大学生物与食品工程学院,合肥 230009;2皖江禽业技术研究院,合肥工业大学宣城校区,宣城 242000
  • 出版日期:2016-06-30 发布日期:2017-03-02

Extraction and Analysis of Mannan from Palm Kernel Expeller

WANG Ren1,NI Jiaojiao1,SHENG Yibao2,CAO Shuqing1,LIU Guoqing2*,FAN Tingting1*   

  1. 1School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China; 2Wanjiang Institute of Poultry Technology,Hefei University of Technology,Xuancheng Campus,Xuancheng 242000,China
  • Online:2016-06-30 Published:2017-03-02

摘要: 本研究从棕榈粕中提取甘露聚糖并对提取物结构进行分析。选取棕榈粕残渣为原料,采用碱提法提取棕榈粕甘露聚糖,并利用紫外光谱、薄层色谱法、红外光谱和1H NMR等实验手段对其产物进行初步分析。得到的试验结果表明:不同浓度氢氧化钠提取率不同,当碱浓度达到10%时甘露聚糖提取率最高,可达到干重的20%左右;紫外光谱分析表明提取物纯度较高;薄层色谱法分析显示提取物的完全酸水解产物为甘露糖;红外光谱和1H NMR光谱分析表明提取出的甘露聚糖多糖特征明显。因此通过本文研究,利用碱提法从棕榈粕中提取的甘露聚糖为βD甘露聚糖。

关键词: 棕榈粕, 甘露聚糖, 提取, 结构分析

Abstract: We extracted the mannan from palm kernel expeller and analyzed its structure in this study.The residue of palm pulp was chosen as raw material and we used adopts alkali method to extract mannan from palm meal.And UV spectrum,thin layer chromatography,infrared spectroscopy,and 1H NMR methods were used to conduct a preliminary analysis of the product.The testing results showed that the different concentration of sodium hydroxide have different extraction rate.When the sodium hydroxide gets 10 %,the concentration of alkali mannan is highest and achieve about 20% of the dry weight;the UV spectrum analysis showed that extraction has high purity;Thinlayer chromatography (TLC) analysis showed that completely acid hydrolysate of mannose was mannose;Infrared spectrum and the 1H NMR spectroscopy analysis showed that the extracted mannan has significantly polysaccharide characteristics.Hence we can draw a conclusion the mannan extracted from palm kernel expeller by using alkali method is βD mannan.

Key words: palm meal, mannan, extract, structural analysis

中图分类号: 

R284.2