复方麦芽丸对多囊卵巢综合征模型大鼠LH、FSH表达的影响

doi:10.16333/j.1001-6880.2017.2.022

天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (2): 304-309.doi: 10.16333/j.1001-6880.2017.2.022

复方麦芽丸对多囊卵巢综合征模型大鼠LH、FSH表达的影响

兰楠1，杨洋博君2，邱文平1，陈蓉1*

1重庆医科大学中医药学院中医药研究室；2重庆市第十三人民医院，重庆 400016

出版日期:2017-02-28 发布日期:2017-03-07

Effects of Compound Malt Pill on the Expression of LH and FSH in Polycystic Ovary Syndrome Model Rats

LAN Nan1,YANG Yang-bojun2,QIU Wen-ping1,CHEN Rong1*

1Traditional Chinese Medicine Research Laboratory,College of Traditional Chinese Medicine,Chongqing  Medical University;2The ThirteenPeople’s Hospital of Chongqing,Chongqing 400016,China

Online:2017-02-28 Published:2017-03-07

摘要/Abstract

摘要： 本文旨在研究复方麦芽丸（Compound Malt Pill，CMP）对多囊卵巢综合征（polycystic ovarian syndrome，PCOS）大鼠黄体生成素（LH）、促卵泡激素（FSH）表达的影响。通过运用9日龄SD大鼠颈背部皮下注射丙酸睾丸酮建立PCOS模型，造模成功的大鼠随机分为模型组，达英-35组和CMP低、中、高剂量组（n=9），另设正常组（n=10）。各治疗组以相应药物灌胃，正常组及模型组灌以等量的蒸馏水。运用免疫组化、PCR测定卵巢组织LH、FSH的蛋白及mRNA表达。结果显示，模型组中LH的蛋白及mRNA表达较正常组显著升高（P<0.01），CMP各剂量组LH蛋白及mRNA表达较模型组显著降低（P<0.01），达英-35组LH蛋白表达较模型组显著降低（P<0.01），mRNA表达较模型组无显著差异（P>0.05）；模型组中FSH的蛋白及mRNA表达较正常组显著降低（P<0.01），各治疗组FSH蛋白及mRNA表达较模型组显著升高（P<0.01），研究结果表明，PCOS模型大鼠LH表达明显升高，FSH表达明显降低；复方麦芽丸对PCOS有治疗作用，可能是通过调节LH、FSH表达水平实现的。

关键词: 多囊卵巢综合征, 复方麦芽丸, 黄体生成素, 促卵泡激素

Abstract: The objective of study was to investigate the effect of compound malt pill(CMP) on the expression of LH and FSH in polycystic ovary syndrome model rats.The 9day-old rats were given the subcutaneous injection of testosterone propionate for inducing PCOS.The model rats were randomly divided into 5 groups(n=9): the model group,Diane 35 group,high,middle,low-dose CMP group.10 rats with normal estrus cycles were set as the normal group.The treatment groups were given intragastric administration with corresponding drugs,the normal group and the model group were given distilled water.The protein and mRNA expression levels of LH and FSH were detected by imunohistochemistry and PCR.The results showed that the protein and mRNA expression of LH were significantly increased in model group than that in normal group (P<0.01),compared with model group,the protein and mRNA expression of LH were significantly decreased in CMP each dose group (P<0.01),the protein expression of LH was significantly decreased in Diane-35 group (P<0.01) and the mRNA expression of LH was not significantly different(P>0.05).The protein and mRNA expression of FSH were significantly decreased in model group than that in normal group (P<0.01),the protein and mRNA expression of FSH were significantly increased in each treatment group than that in model group (P<0.01).Hence,it was concluded that,the protein and mRNA expression of LH was significantly increased and FSH was significantly decreased in PCOS model rats.The therapeutic effect of compound malt Pill on PCOS can be achieved,and its possible mechanism is related to regulating the protein and mRNA expression level of LH and FSH in ovarian tissue.

Key words: compound malt pill, polycystic ovary syndrome, LH, FSH

中图分类号:

R271.19

兰楠1，杨洋博君2，邱文平1，陈蓉1*. 复方麦芽丸对多囊卵巢综合征模型大鼠LH、FSH表达的影响[J]. 天然产物研究与开发, 2017, 29(2): 304-309.

LAN Nan1,YANG Yang-bojun2,QIU Wen-ping1,CHEN Rong1*. Effects of Compound Malt Pill on the Expression of LH and FSH in Polycystic Ovary Syndrome Model Rats[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(2): 304-309.

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复方麦芽丸对多囊卵巢综合征模型大鼠LH、FSH表达的影响

Effects of Compound Malt Pill on the Expression of LH and FSH in Polycystic Ovary Syndrome Model Rats

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