天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (9): 1351-1356.doi: 10.16333/j.1001-6880.2016.9.002

• 研究论文 • 上一篇    下一篇

羊肚菌多糖的分离纯化及其体外抗氧化、抗肿瘤活性研究

李敬1,2*，周伟杰3，赵士豪1,2*，吕慧丽1   

  1. 1 河北经贸大学生物科学与工程学院；2河北经贸大学食品安全管理研究所，石家庄050061；3山东省青岛市黄岛区食品药品监督管理局，青岛266555
  • 出版日期:2016-09-30 发布日期:2017-03-24

Isolation,Purification,Antioxidant and Antitumor Activities of Polysaccharides from Morchella esculenta

LI Jing1,2*,ZHOU Wei-jie3,ZHAO Shi-hao1,2*,LV Hui-li1   

  1. 1 College of Biology Science and Engineering,Hebei University of Economics and Business; 2 Institute of Food Safety Management,Hebei University of Economics and Business,Shijiazhuang 050061,China;  3 Shandong Huangdao Food and Drug Administration,Qingdao 266555,China
  • Online:2016-09-30 Published:2017-03-24

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摘要： 采用DEAE-52柱层析及Sephadex G-200凝胶柱层析从羊肚菌多糖中分离纯化出两种水溶性多糖（MEP 1-1和MEP 2-3）。研究了其分子量、单糖组成、体外抗氧化活性及抗肿瘤活性。结果表明，MEP1-1和MEP2-3分子量分别为1.37×105 Da和1.15×104 Da。红外光谱显示MEP 1-1，MEP 2-3含有少量β-糖苷键。MEP 1-1和MEP2-3抗氧化活性均高于粗多糖，MEP2-3具有较高的羟基自由基活性，而MEP1-1具有较强的清除超氧阴离子自由基活性。MEP 1-1比MEP2-3表现出更高的抑制HeLa活性，IC50值为29 μg/mL。而MEP2-3表现出更高的抑制A549活性，72 h的IC50值为81 μg/mL。

关键词: 羊肚菌, 多糖, 抗氧化活性, 抗肿瘤活性

Abstract: Two water-soluble polysaccharides (MEP1-1 and MEP2-3) were extracted and purified using chromatography on DEAE-52 and Sephadex G-200 column from Morchella esculenta. Their chemical composition and in vitro antioxidant and antitumor activities were investigated.The results suggested that the molecular weights of MEP 1-1 and MEP 2-3 were approximately 1.37×105 Da and 1.15×104 Da,respectively.Characteristic bands in FT-IR spectra indicated both MEP1-1 and MEP2-3 had a small amount of β-glycosidic bond.Antioxidant activities of MEP1-1 and MEP2-3 were both higher than crude MEP.MEP2-3 had higher antioxidant activities on hydroxyl radicals than MEP1-1 while MEP1-1 had higher antioxidant activities on superoxide radicals than MEP2-3.MEP 1-1 fraction appeared higher anticancer activity against HeLa cells than MEP2-3 fraction,with IC50 values of 29 μg/mL.MEP2-3 fraction showed higher anticancer activity against A549 cells than MEP 1-1 fraction,with IC50 values of 81 μg/mL at 72 h.It could be explored as a potential adjuvant against cancer used in the health food and pharmaceutical therapy.

Key words: Morchella esculenta, polysaccharides, antioxidant activity, antitumor activity

中图分类号: 

Q946.3

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