天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (10): 1549-1556.doi: 10.16333/j.1001-6880.2016.10.009

• 研究简报 • 上一篇    下一篇

宁夏野生甘草中产甘草黄酮耐盐内生真菌分离及其DPPH自由基清除作用

高晓娟1,2,3,吴秀丽1,2,吴玉玲1,陈靖1,2,3*   

  1. 1 药学院 宁夏医科大学;2宁夏回药现代化工程技术研究中心;3宁夏医科大学回医药现代化省部共建教育部重点实验室,银川 750004
  • 出版日期:2016-10-31 发布日期:2017-03-30

Separation of Licorice Flavonoids Producing Salt-resistant Endophytic Fungi from Wild Glycyrrhiza uralensis Fisch Living in Ningxia District

GAO Xiao-juan1,2,3,WU Xiu-li1,2,WU Yu-ling1,CHEN Jing1,2,3*   

  1. 1 School of Pharmacy,Ningxia Medical University;2Ningxia Research Center of Modern Hui Medicine  Engineering and Technology;3 Key Laboratory of Hui Ethnic Medicine Modernization,Ministry of Education,Ningxia  Medical University,Yinchuan 750004,China
  • Online:2016-10-31 Published:2017-03-30

摘要: 采用组织分离法,在不含盐及含盐PDA培养基上分离、纯化内生菌,以甘草苷等8种甘草黄酮为对照品,分析发酵产物;DPPH自由基清除法测定清除率。共得到108株内生菌,TLC发现31株可能产甘草黄酮;HPLC-UV发现产甘草苷7株,产异甘草苷3株,产甘草素3株,产甘草查尔酮A3株,产刺甘草查尔酮1株,均为耐盐菌;产甘草苷、异甘草苷、甘草查尔酮A、刺甘草查尔酮的部分菌株有较强DPPH清除率;产甘草苷和异甘草苷的菌株8-5-Y-2活性最强,与甘草总黄酮相当,强于甘草查尔酮A。盐协迫得到产甘草黄酮,且具较强DPPH自由基清除活性的耐盐内生真菌,为资源替代奠定基础。

关键词: 甘草, 耐盐内生真菌, 甘草黄酮, HPLC-UV, DPPH

Abstract: The PDA medium containing different concentrations of NaCl was used to isolate the salt-resistant endophytic fungi from wild Glycyrriza uralensis.The extracts were analyzed by TLC and HPLC-UV methods to screen the licorice flavonoids-producing fungi.Totally 108 fungi were obtained and 31 strains were proved to possibly produce licorice flavonoids by TLC.Analyzed by a HPLC-UV method,7 strains of fungi were manifested to produce liquiritin and 3 strains produced isoliquiritin.3 strains produced glycyrrhizin and 3 strains were observed to produce licochalcone A.Finally,the strain named 8-2-G-3 was found to produce echinatin.The DPPH free radical scavening assay results showed that some flavonoids-producing strains,such as liquiritin,isoliquiritin,licochalcone A,and echinatin,had strong antioxidant activities.The strain 8-5-Y-2,which produced liquiritin and isoliquiritin,had a strong activity euqal to total licorice flavonoids while stronger than licochalcone A.Salt-resistant culture was beneficial to obtain the licorice flavonoids-producing salt-resistant fungi which had strong DPPH free radical scavening activity.

Key words: Glycyrrhiza uralensis, salt-resistant endophytic fungi, licorice flavonoids, HPLC-UV, DPPH free radical scavening

中图分类号: 

R932