“内异消”对子宫内膜异位症大鼠CYP1A2、CYP2E1、CYP3A4活性的影响

doi:10.16333/j.1001-6880.2016.10.024

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (10): 1627-1632.doi: 10.16333/j.1001-6880.2016.10.024

“内异消”对子宫内膜异位症大鼠CYP1A2、CYP2E1、CYP3A4活性的影响

冯彬彬1,2，张建海1,2*，徐晓玉3

1 重庆三峡医药高等专科学校；2重庆市抗肿瘤天然药物工程技术研究中心，重庆404120；3西南大学，重庆 400716

出版日期:2016-10-31 发布日期:2017-03-30

Effect of Neiyixiao on Hepatic CYP1A2,CYP2E1,CYP3A4 in Rats with Endometriosis

FENG Bin-bin1,2,ZHANG Jian-hai1,2*,XU Xiao-yu3

1Chongqing Three Gorges Medical College;2Chongqing Engineering Research Center of Antitumor Natural Drugs,Chongqing 404120,China;3Southwest University,Chongqing 400716,China

Online:2016-10-31 Published:2017-03-30

摘要/Abstract

摘要： 本文主要考察“内异消”对子宫内膜异位症大鼠肝微粒体CYP1A2、CYP2E1、CYP3A4活性的影响。将16只SD大鼠随机分为空白对照组、正常给药组，16只模型SD大鼠随机分为模型对照组和模型给药组。正常给药组和模型给药组每天灌胃给予“内异消”，空白对照组和模型对照组灌胃给予羧甲基纤维素钠溶液，均连续给药7 d，于第8 d给予“Cocktail”探针药物咖啡因、氯唑沙宗和硝苯地平药物，通过HPLC检测Cocktail探针药物的代谢率评价各组CYP450及CYP1A2、CYP2E1、CYP3A4活性。结果表明，与正常组比较，模型对照组和模型给药组咖啡因、氯唑沙宗的t_1/2减小，硝苯地平的t_1/2延长，均无显著性差异（P>0.05）；与正常组比较，模型对照组和模型给药组咖啡因、氯唑沙宗的AUC(0-t) 显著升高（P<0.05），硝苯地平的AUC(0-t) 的增加无显著性。说明“内异消”对子宫内膜异位症大鼠CYP1A2、CYP2E1、CYP3A4的活性不存在显著的相互作用。

关键词: 内异消, 细胞色素P450, 肝微粒体

Abstract: To observe the effects on the activity CYP 1A2,CYP2E1 and CYP3A4 in model rats of endometriosis following administration “Neiyixiao”.16 Sprague-Dawley rats were randomly divided into 2 groups:normal control group and normal administration group.16 Sprague-Dawley model rats were randomly divided into 2 groups:model control group and model administration group.All the rats of control group were given CMC-Na and all the rats of administration group were given “Neiyixiao” for seven days.After eight days,the plasma concentrations of Cocktail probe drugs of CYP1A2,CYP2E1 and CYP3A4 were determined by HPLC.Compared with normal control group,the level of t_1/2 of caffeine and chlorzoxazone in model control group and model administration group had diminished (P>0.05),the level of t_1/2of nifedipine had prolonged (P>0.05).The level of AUC(0-t) of caffeine and chlorzoxazone in model control group and model administration group was increased significantly (P<0.05),the level of AUC(0-t) of nifedipine was not significant.The results showed that "Neiyixiao" cannot interact with CYP 1A2,CYP2E1 andCYP3A4 in model rats of endometriosis.

Key words: Neiyixiao, CYP450, liver microsome

中图分类号:

R285.5

冯彬彬1,2，张建海1,2*，徐晓玉3. “内异消”对子宫内膜异位症大鼠CYP1A2、CYP2E1、CYP3A4活性的影响[J]. 天然产物研究与开发, 2016, 28(10): 1627-1632.

FENG Bin-bin1,2,ZHANG Jian-hai1,2*,XU Xiao-yu3. Effect of Neiyixiao on Hepatic CYP1A2,CYP2E1,CYP3A4 in Rats with Endometriosis[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(10): 1627-1632.

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“内异消”对子宫内膜异位症大鼠CYP1A2、CYP2E1、CYP3A4活性的影响

Effect of Neiyixiao on Hepatic CYP1A2,CYP2E1,CYP3A4 in Rats with Endometriosis

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