日粮添加灰毡毛忍冬藤叶粉对肥育猪血清生化指标与脂肪代谢的影响

doi:10.16333/j.1001-6880.2016.11.023

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (11): 1810-1815.doi: 10.16333/j.1001-6880.2016.11.023

日粮添加灰毡毛忍冬藤叶粉对肥育猪血清生化指标与脂肪代谢的影响

王勤华1,2，龙次民2，周锡红2，谢春艳2，李凤娜2，范志勇1*，印遇龙2，吴信1,2*

1 湖南农业大学湖南畜禽安全生产协同创新中心，长沙 410128；2中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室，湖南省畜禽健康养殖工程技术研究中心，长沙 410125

出版日期:2016-11-30 发布日期:2017-03-30

Effects of Dietary Supplementation of Lonicera macranthoides Leaves on Blood Biochemical Indexes and Fat Metabolism of Growing-finishing Pigs

WANG Qin-hua1,2,LONG Ci-min2,ZHOU Xi-hong2,XIE Chun-yan2,LI Feng-na2,FAN Zhi-yong1*,YING Yu-long2,WU Xin1,2*

1 Hunan Co-Innovation Center of Animal Production Safety,Hunan Agricultural University,Changsha 410128,China; 2 Key Laboratory for Agro-ecological Processes in Subtropical Region and Hunan Engineering and Research Center of Animal and Poultry Science,Institute of Subtropical Agriculture, The Chinese Academy of Sciences,Changsha 410125,China

Online:2016-11-30 Published:2017-03-30

摘要/Abstract

摘要： 本试验旨在研究日粮中添加灰毡毛忍冬藤叶粉对肥育猪血清生化、肝脏中脂肪酸组成以及脂肪代谢相关基因表达的影响。选用同栏舍、同批次的二元杂交（长×大）健康阉公猪（55±1.03 kg）16栏，按照体重相近原则随机分成2个处理组，即基础日粮（对照组）、基础日粮+1%灰毡毛忍冬藤叶粉，每组8个重复，每个重复10头。试验期为60 d。结果表明：1）与对照组相比，日粮添加灰毡毛忍冬藤叶粉降低了血清中总胆固醇（TC）（P<0.05），而显著提高C型反应蛋白（CRP）含量（P<0.05）；2）与对照组相比，日粮中添加灰毡毛忍冬藤叶粉使肝脏中饱和脂肪酸（SFA）中c13:0和单不饱和脂肪酸（MUFA）中的c14:1含量均显著降低（P<0.05）；3）与对照组相比，日粮中添加灰毡毛忍冬藤叶粉上调了肝脏中甘油三酯脂肪酶（ATGL）和肉碱棕榈酰基转移酶1（CPT1A）的基因表达量（P<0.05）；而下调了脂肪合成酶（FAS）的基因表达量（P<0.10）。综上所述，日粮中添加灰毡毛忍冬藤叶粉能有效降低肥育猪血液中胆固醇和甘油三酯的含量，可能通过调控脂肪代谢基因ATGL、CPT1和FAS的表达，进而调控育肥猪肝脏的脂肪代谢。

关键词: 灰毡毛忍冬, 生化指标, 脂肪酸组成, 脂肪代谢, 肥育猪

Abstract: The aims of this study was to determine the effects of dietary supplementation of Lonicera macranthoides leaves(LML) on blood biochemical indexes,liver fatty acid composition and the expression of fat metabolism-related genes in finishing pigs.A total of 160 crossbred barrows (Large White × Landrace) with an average initial BW of 55±1.03 kg were randomly allocated to 2 treatment groups (each treatment was replicated eight times with ten pigs each) and fed either a basal diet or a diet supplemented with 1.0% LML powder.The experiment lasted for 60 days.The results were showed as follows:1) Compared with the control group,dietary supplementation of LML powder significantly decreased the serum total cholesterol (TC) content (P<0.05) and increased the c-reaction protein (CRP) content (P<0.05);2) Compared with the control group,dietary supplementation of LML powder significantly decreased the liver content of SFA c13:0 and MUFA of c14:1 (P< 0.05);3) Compared with the control group,the mRNA expression levels of Adipose trigtyceride lipase (ATGL) and Carnitine acyl transferase-1 (CPT1A) genes in the dietary supplementation of LML powder were significantly up-regulated (P<0.05);while the mRNA expression levels of Lipogenic enzymes (FAS) genes were significantly down-regulated (P<0.10).In conclusion,dietary supplementation of LML powder can effectively reduce the content of serum total triglyceride (TG) and total cholesterol (TC) in finishing pigs and these effects may be associated with the expression of fat metabolism-related genes,thus regulating the liver fat metabolism.

Key words: Lonicera macranthoides, biochemical index, fatty acid composition, fat metabolism, finishing pigs

中图分类号:

S828.5

王勤华1,2，龙次民2，周锡红2，谢春艳2，李凤娜2，范志勇1*，印遇龙2，吴信1,2*. 日粮添加灰毡毛忍冬藤叶粉对肥育猪血清生化指标与脂肪代谢的影响[J]. 天然产物研究与开发, 2016, 28(11): 1810-1815.

WANG Qin-hua1,2,LONG Ci-min2,ZHOU Xi-hong2,XIE Chun-yan2,LI Feng-na2,FAN Zhi-yong1*,YING Yu-long2,WU Xin1,2*. Effects of Dietary Supplementation of Lonicera macranthoides Leaves on Blood Biochemical Indexes and Fat Metabolism of Growing-finishing Pigs[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(11): 1810-1815.

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日粮添加灰毡毛忍冬藤叶粉对肥育猪血清生化指标与脂肪代谢的影响

Effects of Dietary Supplementation of Lonicera macranthoides Leaves on Blood Biochemical Indexes and Fat Metabolism of Growing-finishing Pigs

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