关键词: 恒山黄芪, 多糖, 肿瘤细胞, 粘附, 铺展, 迁移

Abstract: To evaluate the effects of Hengshan Astragalus polysaccharides on human cervical cancer cell SiHa,MTT assay was used to determine cell proliferation and viability of SiHa.The cell adhesive assay and cell spreading assay were used to detect the adhesive and spreading capabilities of SiHa,and the woundhealing assay was used to access the migration capability of SiHa.The results indicated that Hengshan Astragalus polysaccharides showed significant activity of suppressing SiHa cell proliferation (IC₅₀ value was 0.25 mg/mL) even at a low concentration (0.0625 mg/mL).Compared with the control group,the adhesive and spreading capability of SiHa cells treated with Hengshan Astragalus polysaccharides were obviously reduced (P<0.01),and the migration capability was also depressed remarkably (P<0.01).Therefore,polysaccharides from the genuine medicinal material Hengshan Astragalus can inhibit the proliferation,adhesion,spreading and migration capability of human cervical cancer cell SiHa.

Key words: Hengshan Astragalus, polysaccharides, cancer cells, cell adhesion, cell spreading, cell migration