关键词: 细辛, 苯丙素, 甲基丁香酚, 榄香素, 黄樟醚, 生物信息学分析, 实时荧光定量PCR

Abstract: In order to explore the expression level of enzyme genes relevant to the biosynthesis of phenylpropanoids in Asarum, the enzyme genes relevant to the biosynthesis of phenylpropanoids were screened out according to the gene functional annotation which was based on the database of Asarum transcriptome constructed in the laboratory. The protein sequence was deduced and was analyzed by bioinformatics. Meanwhile,expression analysis of the enzyme genes was carried out. Six enzyme genes were screened out from the transcriptome of Asarum, including pal, c4h, comt, 4cl, ccr and cad. The protein sequence encoded by six enzyme genes had different superfamily. The expression level of six enzyme genes showed the same changes. For leaf,petiole,root and rhizome,the expression level of six enzyme genes in flowering stage was higher than that in early flowering stage,and the expression level of six enzyme genes in the underground part was higher than that in the aboveground part.The above results showed thatthe expression level of six enzyme genes in leave,petiole,root and rhizome of Asarum was different,but there was a certain regularity. The success of this study will lay a foundation for further understanding of the functions and characteristics of the enzymesrelevant to the biosynthesis of phenylpropanoids,and provide a theoretical basis for future biosynthesis and metabolic regulation of active ingredients in Asarum.

Key words: Asarum, phenylpropanoid , methyl eugenol, elemene, safrole, bioinformatics analysis, quantitative real-time PCR